摘要
从取材方式、灭菌前预处理、培养基添加抗褐变剂3方面研究降低南板蓝根芽体褐变的方法。结果表明:掰芽取材和及时流水冲洗处理对减轻南板蓝根芽体褐变有显著的效果;培养基中添加0.5%活性炭吸附剂对减轻褐变有一定作用,添加抗氧化剂柠檬酸300～500 mg/L浓度对褐变的抑制效果不明显。掰取芽体后立即流水冲洗2 h,培养基中添加0.5%活性炭,能有效减轻芽褐变和利于快速增殖。
Shoot browning is a serious phenomenon during rapid propagation of Baphicacanthus cusia （Nees）Bremek. This study attempts to control browning of shoot by three methods in order to increase the shoot ability of rapid propagation in vitro. The results showed that the browning degree of shoots which are broken off from plant and then flushed several times by water were lighter than the browning degree of shoots which were cut off by tool;The appropriate concentration of activated carbon 0. 5 % in medium may reduce the shoot browning in some degree,but concentration of citric acid 300- 500 mg· L^-1 had no effect significantly. In conclusion,the method that shoots were broken off from plant and flushed 2 hours by water,then explanted the medium adding 0. 5% activated carbon,can decrease the browning effectively.
出处
《北方园艺》
CAS
北大核心
2010年第3期123-125,共3页
Northern Horticulture
基金
广州中医药大学中医药科研创新基金资助项目(09CX018)
广州大学本科生创新性实验资助项目
关键词
南板蓝根
芽
褐变
Ba ph icacanthus cusia （Nees）Bremek
shoot
browning
