摘要
目的观察枸杞多糖对效应细胞的增殖和杀瘤活性的作用。方法通过密度梯度离心法分离人外周血单核细胞并培养其为树突状细胞,用冻融法制备肝癌细胞的全抗原并致敏树突状细胞;通过混合淋巴细胞实验,获取树突状细胞激活的特异性效应T细胞。用MTT法测定效应T细胞对肝癌细胞的杀伤率。观察枸杞多糖在树突状细胞的成熟及T细胞的激活、增殖和杀瘤过程中的作用。结果枸杞多糖单独对T细胞无明显的增殖能力,但能促进树突状细胞的成熟并且能增强效应T细胞的增殖能力和杀瘤活性。结论枸杞多糖能促进树突状细胞成熟和增强效应T细胞的增殖和杀伤肿瘤的活性。
Objective To investiage the proliferation and anti-tumor of effective T cells by lycium bararum polysaccharides Methods Monocytes obtained form human peripheral blood by density guadient centrifugation are induced into DCs.The whole antigen of hepatocarcinoma cells are extracted by freeze thawing method and sensitize DCs;we harvest effective T cells by mixed lymphology reaction,we examine the specificity that effective T cells kill target hepatocarcinoma cells by the MTT method.The effection of lycium bararum polysaccharides on DCs＇ maturity,T cell activation,proliferation and anti-tumor are observed.Results Lycium bararum polysaccharides alone has no obvious effection on the proliferation of T cells but they can promote the maturity of DCs and reinforce proliferation and anti-tumor of effective T cells.Conclusion Lycium bararum polysaccharides can promote the maturity of DCs and reinforce proliferation and anti-tumor of effective T cells.
出处
《中国实用医药》
2010年第4期21-22,共2页
China Practical Medicine
关键词
枸杞多糖
树突状细胞
抗肿瘤
T细胞
Lyeium bararum polysaccharides
Dendritic Cell
Anti-tumor
T cells
