摘要
目的探讨凋亡抑制基因Survivin和增殖细胞核抗原(PCNA)在自然杀伤(NK)/T细胞淋巴瘤中的表达及其关系。方法NK/T细胞淋巴瘤标本25例,经过组织学形态及CD45RO、CD3ε、CD20免疫组织化学分析,免疫组织化学链霉素抗生物素-过氧化酶连接法(SP法)检测T细胞内抗原(TIA-1)、粒酶B,原位杂交法检测EB病毒编码的mRNA(EBER 1/2),按照2000年WHO新的淋巴瘤分类方法明确诊断。应用SP法检测25例NK/T细胞淋巴瘤组织和10例反应性增生淋巴组织中的Survivin和PCNA表达水平,分别计数1 000个肿瘤细胞中Survivin及PCNA阳性细胞所占百分比,作为Survivin阳性率及细胞增殖指数(PI)。结果25例NK/T细胞淋巴瘤组织中Survivin表达阳性17例(68.0%),10例反应性增生淋巴组织中Survivin不表达,其差异有统计学意义(P<0.01);25例NK/T细胞淋巴瘤PI为(41.48±5.10)%,10例反应性增生淋巴组织PI为(20.10±2.77)%,二者比较差异有统计学意义(t=12.39,P<0.01);Survivin表达阳性组PI为(43.35±4.80)%,显著高于阴性组[(37.50±2.90)%](t=3.16,P<0.01);Survivin表达与平均PI呈正相关(r=0.637,P<0.01)。结论NK/T细胞淋巴瘤中Survivin基因和PCNA表达上调,Survivin可能通过调控细胞增殖参与NK/T细胞淋巴瘤的发生发展。
Objective To investigate the expressions of Survivin gene and proliferation cell nuclear antigen（PCNA） in natural killer（NK）/T cell lymphoma and its relationship.Methods Twenty-five cases of NK/T cell lymphoma underwent histomorphological and CD45RO,CD3ε,CD20 immunohistochemical analysis.Immunohistochemical SP method was used to detect T cell intracellular antigen-1（TIA-1）and granzyme B,insituhybridization（ISH） method was used to detect mRNA encoding EBV（EBER 1/2）.The diagnosis was based on WHO classification system（2000）.Immunohistochemistry was used to study the expressions of Survivin,PCNA in 25 cases NK/T cell lymphoma and 10 cases reactive lymphoid tissues.The number of positive cells in every 1 000 tumor cells was counted as the Survivin positive rate,proliferative index（PI）,respectively.Results Survivin expression was positive in 17 cases NK/T cell lymphomas（68.0%）,while no expression of Survivin was detected in the 10 cases reactive lymphoid tissues（P〈0.01）.PI averaged was（41.48±5.10）% in NK/T cell lymphoma and（20.10±2.77）% in the 10 cases reactive lymphoid tissues respectively,and the difference was significant（t=12.39,P〈0.01）.PI in NK/T cell lymphoma with positive expression of Survivin was significantly higher than that with negative Survivin [（43.35±4.80）% vs（37.50±2.90）%],the difference was significant（t=3.16,P〈0.01）.Survivin and PI was positively related（r=0.637,P〈0.01）.Conclusions Survivin gene and PCNA expression are up-regulated in NK/T cell lymphoma.Survivin gene may play a role in the pathogenesis of NK/T cell lymphoma tumor by influencing cell proliferation.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第3期172-174,共3页
Journal of Applied Clinical Pediatrics
基金
湖北省教育厅重点科研项目(B200624014)
