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壳寡糖诱导烟草防御酶系活性变化及PR-1a基因表达研究 被引量:11

Changes of defensive enzymes and PR-1a gene expression of tobacco induced by chito-oligosaccharides
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摘要 The activity change of defensive enzymes and PR-1a gene expression of tobacco (Nicotiana tabacum) seedling induced by chito-oligosaccharides were studied. The results showed that high level systemic acquired resistance (SAR) was expressed in tobacco plants treated with chito-oligosaccharides solution at the concentration of 50 μg/mL. PAL activity increased greatly with 2 peaks, the activity of SOD decreased initially followed by an increase with higher increment, and the activity of POD peaked early followed by a gentle fall in chito-oligosaccharide treated plants. The PR-1a gene was strongly expressed in tobacco due to systemic acquired resistance induced by chito-oligosaccharides. At 168 h after inoculation the expression quantity (co-pies/2 μL) of PR-1a gene was increased to 2 469.6 in treated tobacco leaf, reached 392.6% than that at 0 h after inoculation,it was increased 3.05 times of that in untreated control. The activity change of defensive enzymes and PR-1a gene expression of tobacco (Nicotiana tabacum) seedling induced by chito-oligosaccharides were studied. The results showed that high level systemic acquired resistance (SAR) was expressed in tobacco plants treated with chito-oligosaccharides solution at the concentration of 50 μg/mL. PAL activity increased greatly with 2 peaks, the activity of SOD decreased initially followed by an increase with higher increment, and the activity of POD peaked early followed by a gentle fall in chito-oligosaccharide treated plants. The PR-1a gene was strongly expressed in tobacco due to systemic acquired resistance induced by chito-oligosaccharides. At 168 h after inoculation the expression quantity (co-pies/2 μL) of PR-1a gene was increased to 2 469.6 in treated tobacco leaf, reached 392.6% than that at 0 h after inoculation,it was increased 3.05 times of that in untreated control.
出处 《植物病理学报》 CAS CSCD 北大核心 2010年第1期99-102,共4页 Acta Phytopathologica Sinica
基金 教育部长江学者和创新团队发展计划(200558) 高等学校学科创新引智计划(B07049) 西北农林科技大学博士启动基金资助
关键词 活性变化 防御酶系 壳寡糖 基因表达 烟草 诱导 系统获得抗病性 植物病毒 chito-oligosaccharides SAR defensive enzyme PR-1a gene
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