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实时荧光定量PCR技术原理与应用 被引量:34

Principles and Application of Real-time Fluorescent Quantitative PCR Technique
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摘要 实时荧光定量PCR(real-ti me fluorescent quantitative polymerase chain reaction,FQ-PCR)是在定性PCR技术基础上发展起来的核酸定量技术,它具有特异性强、PCR污染少、自动化程度高等特点。作者就实时荧光定量PCR技术的主要原理及目前在分子生物学领域,病原体和肿瘤等方面的检测和诊断应用进行了概述。 Real-time quantitative PCR is a qualitative PCR technique developed on the basis of the nucleic acid quantification.The qualities to be more specific,less polluted,and highly automated.This paper reviews main principles,and detection and diagnosis in pathogens and tumors of real-time fluorescence quantitative PCR.
出处 《中国畜牧兽医》 CAS 北大核心 2010年第2期87-89,共3页 China Animal Husbandry & Veterinary Medicine
基金 国家自然科学基金(30771601) 河南省杰出人才创新基金(0521001800) 河南省高校科技创新人才支持计划(2009HASTIT010)
关键词 实时荧光定量PCR 原理 检测 应用 real-time quantitative PCR principles detection application
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参考文献10

  • 1冯文曦,曹红卫,赵静怡,郑红,钟白玉.应用荧光定量PCR技术检测淋球菌等性传播疾病病原微生物[J].第三军医大学学报,2002,24(11):1357-1357. 被引量:3
  • 2刘园园,吴晖,肖性龙,贾赟,吴斌,余以刚.猪伪狂犬病病毒TaqMan-MGB荧光定量PCR检测方法的建立及应用[J].中国畜牧兽医,2009,36(4):76-79. 被引量:11
  • 3赵卫东,祝怀平,凌斌,孙敏文,陈纲,王群华,朱园园,陈敏.荧光定量PCR法检测肿瘤细胞株转录因子表达水平[J].临床输血与检验,2004,6(2):86-88. 被引量:3
  • 4Andersen C L,Jeusen J L, Orntoft T F. Normalisation of real- time quantitative reverse transcription PCR data: a model based variance estimation approach to identify genes suited for normalization applied to bladder and colon cancer data sets[J]. Cancer- Res,2004,64:5245-5250.
  • 5Cassinat B, Zassadowski F, Balitrand N, et al. Quantitation of minimal residual disease in acute promyelocytic leukemia patients with t (15:17) translocation using real-time RT PCR. Leukemia, 2000,14:324-328.
  • 6Ian M M,Katherine E A, Andreas N. Survey and summary real-time PCR in virology[J]. Nucleic Acids Res,2002,30(6) :1292- 1305.
  • 7James A H, Ron F, et al, Real time PCR for the detection of Cryptosporidium parvum[J]. Journal of Microbiological Metrods, 2000,147:323-337.
  • 8Pongers Willemse M J, Verhagen O J ,Tibbe G J ,et al. Real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia using junctional region specific TaqMan probes. Leukemia, 1998,12: 2006-2014.
  • 9Straubinger R K. PCR based quantification of Borrelia burg dorferi organisms in canine tissues over a 500-day postin{ection period[J]. J Clin Microbiol,2000,38(6) :2191 -2199.
  • 10Thiery R, Boutin P, Arnauld C, et al. Pseudorabies virus latency: a quantitative app roach by polymerase chain reaction[J]. Acta Veterinaria Hungarican,1994,42(2) :277-287.

二级参考文献16

  • 1赵丽,崔保安,方忠意,李小康,陈红英,夏平安,朱瑞芳.PCR检测猪伪狂犬病病毒方法的研究[J].中国预防兽医学报,2007,29(2):142-146. 被引量:23
  • 2管宇,沈志强,曲光刚,吕素芳,任艳玲,李书光,王玉炯.猪伪狂犬病病毒SA株的分离鉴定及其gE基因缺失株转移载体的构建[J].中国畜牧兽医,2007,34(6):109-111. 被引量:7
  • 3邹先进 罗凯 等.实时荧光定量聚合酶链反应检测沙眼衣原体等病原体感染[J].中华检验医学杂志,2000,23(2):86-86.
  • 4Brown T M, Osorio F A, Rock D L. Detection of latent pseudorabies virus in swine using in si tu hybridization [J]. Vet Microbiol, 1990,24:273-280.
  • 5Clegg R M. Fluorescence resonance energy transfer [J]. CurrOp in Biotechnol, 1995, 6 (1):103-110.
  • 6Gutekunst D E, Pirtle E C, Miller L D,et al. Isolationof pseudorabies virus from trigeminal ganglia of a latently infected sow [J].Am J VetRes,1980,41(8):1315-1316.
  • 7Maes R K, Sussman M D, Vilnis A, et al. Recent development s in latency and recombination of pseudorabies virus [J].Vet Microbiol, 1997,55 : 13-27.
  • 8Vhiery R, Boutin P, Arnauld C,et al. Pseudorabies virus latency: a quantitative approach by polymerase chain reaction[J].Acta Vet Hung,1994,42:277-287.
  • 9Annapula G,Lut O. An overview of real-time PCR:application to quantify cytokine gene expression [J].Method, 2001,25 (9) : 386-401.
  • 10Zheng WP, Flavell RA. The transciption factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4-T cells[J]. Cell, 1997,89 (5) : 587-597.

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