摘要
结合多维液相色谱和串联质谱分析,iTRAQ技术已成为差异蛋白质组学定量研究的主要工具之一。而寻找和发现区别于正常生理状态下的疾病特异表达蛋白质,有利于阐明疾病的发病机理,对疾病的预防、诊断、预后和疗效监测具有重要作用,并有助于用作新靶点来开发临床治疗药物。本文重点就该技术在医学领域中进行差异蛋白质组分析并寻找标记蛋白质的研究进行综述。
Combined with multi-dimensional liquid chromatography and tandem mass spectrometry, isobaric tags for relative and absolute qnantitation （iTRAQ） has been one of the main technologies for quantitatively comparative proteomic study. Search and discovery for potential disease-specific biomarkers differentiating from physiological processes can assist to eluci- date pathogenesis and facilitate the prevention, diagnosis, prognosis and treatment of diseases, as well as to develop new drug targets for clinical use. Herein, we summarize its application in biomarker discovering in comparative proteomic studies in medical fields.
出处
《生命的化学》
CAS
CSCD
北大核心
2010年第1期135-140,共6页
Chemistry of Life
关键词
ITRAQ
差异蛋白质组分析
蛋白质标记
isobaric tags for relative and absolute quantitation （iTRAQ）
quantitatively comparative proteomic
biomarker
