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4种鼠疫F1抗原/抗体检测方法的初步评价 被引量:8

Preliminary evaluation of four methods to determine the F1 antigen/antibody of plague
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摘要 目的客观评价血凝试验、胶体金试验、ELISA和PCR4种检测方法的灵敏性。方法对于血清学方法中的F1抗体检测,评价检出的全菌免疫兔抗血清最低稀释度;F1抗原检测,评价检出的最低浓度。对于分子生物学方法,评价同时检出fra基因和pla基因的模板最低浓度。结果对于F1抗体检测,间接血凝试验检出的最低稀释度是1∶64,胶体金试验是1∶1000,ELISA是1∶204800。对于F1抗原检测,反向血凝试验检出的最低浓度是2ng/ml,胶体金试验是50ng/ml。fra、pla基因同时检出,模板的最低浓度是0.21ng/μl。结论在以抗体IgG为主的血清中,ELISA法对于F1抗体检测的灵敏性较高。反向间接血凝试验对于F1抗原检测的灵敏性较高。为减少误诊率,采用PCR诊断鼠疫菌要求最低模板浓度是0.21ng/μl。 Objective To evaluate the sensitivity of the four test methods, hemagglutination test, gold immunochromatographic assay (GICA), enzyme-linked immune sorbent assay (ELISA) and polymerase chain reaction (PCR). Methods The minimal detectable dilution of antiserum of a bacteria-immunity rabbit via serological approaches for F1 antibody detection, the minimal detectable concentration in F1 antigen measurement, and the minimal concentration of template with a detectable gene pair,fra and pla, were evaluated. Results For the Fl antibody test, the minimal dilution detected was 1:64 through IHA, 1:1000 through GICA, and 1 : 204 800 through ELISA. For FI antigen test, the minimal detectable concentration was 2 ng/ml by RIHA and 50 ng/ml by GICA. The minimal concentration of template was 0.21 ng/μl whenfra and pla were both detected. Conclusion In the serum consisting mainly of antibody IgG, the ELISA method has a higher sensitivity detecting F1 antibodies. The RIHA has higher sensitivity detecting FI antigens. To minimize misdiagnosis, a minimal concentration of template of 0.21 ng/μl is required to diagnose plague when using PCR.
出处 《中国媒介生物学及控制杂志》 CAS CSCD 2010年第1期39-42,共4页 Chinese Journal of Vector Biology and Control
关键词 鼠疫耶尔森菌 血凝试验 胶体金试验 酶联免疫吸附试验 聚合酶链反应 Yersinia pestis Hemagglutination test Gold immunochromatographic assay Enzyme-linked immune sorbent assay Polymerase chain reaction
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