摘要
[目的]探讨多胺增强植物抗旱性的机理,为多胺作为外源生长调节物质在玉米抗旱过程中的应用提供理论基础。[方法]以聚乙二醇(PEG-6000)模拟自然干旱,用外源Spd处理抗旱性不同的2个玉米品种农大108和掖单13,检测渗透胁迫下2个品种幼苗叶片中相对含水量及可溶性蛋白含量的变化。[结果]渗透胁迫7 d,抗旱性弱的掖单13玉米幼苗叶片的相对含水量和可溶性蛋白含量的下降幅度明显大于抗旱性较强的品种农大108;外源Spd处理明显抑制在胁迫条件下掖单13幼苗叶片相对含水量的下降,并且提高了其在胁迫条件下可溶性蛋白的含量。[结论]外源Spd处理,通过提高渗透胁迫下玉米幼苗叶片内蛋白质的含量,提高了玉米幼苗的抗渗透胁迫能力。
[Objective] The research aimed to probe into the mechanism of polyamine enhancing the tolerance of plants to drought stress and provide theoretical basis for application of polyamine in process of maize drought resistance.[Method]With PEG-6000 simulating natural drought,the change in content of soluble protein and relative water content were investigated in seedling leaves of two maize cultivars,Nongda 108 and Yedan 13 under osmotic stress with exogenous Spd treatment.[Result] On the 7th day,leaf relative water content and the content of soluble protein decreased more significantly in leaves of Yedan 13（drought-sensitive） than in Nongda 108（drought-tolerant）.Exogenous Spd treatment not only obviously inhibited the decrease of leaf relative water content,but also increased the content of soluble protein.[Conclusion] Exogenous Spd treatment could enhance the tolerance of maize seedlings to osmotic stress,via improving the content of soluble protein in seedling leaves.
出处
《安徽农业科学》
CAS
北大核心
2010年第5期2292-2293,共2页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30771296)
河南省基础与前沿技术研究计划项目(082300430310)
河南省教育厅自然科学基础研究计划项目(2007180052)
关键词
渗透胁迫
玉米
多胺
可溶性蛋白
Osmotic stress
Maize
Polyamine
Soluble protein
