摘要
共用３８０个１０碱基随机引物，对抗条锈病基因ＹｒＬｅｅ的近等基因系进行了ＲＡＰＤ分析，其中有两个引物在抗病的近等基因系ＹｒＬｅｅ／６＊Ｔａｉｃｈｕｎｇ２９Ｆ３和基因供体亲本“Ｌｅｅ”中扩增出了特异的ＤＮＡ片段，而在轮回亲本“Ｔａｉｃｈｕｎｇ２９”中没有扩增出同样的ＤＮＡ片段。引物ＯＰＲ－７扩增出的ＤＮＡ片段长度约为４６５ｂｐ，引物ＯＰＲ－９扩增出的ＤＮＡ片段长度约为６６７ｂｐ。用基因供体亲本“Ｌｅｅ”与感病品种“铭贤１６９”杂交的Ｆ２代分离群体（１８９株植株）进行的遗传连锁性分析表明，引物ＯＰＲ－７扩增出的特异ＤＮＡ片段与ＹｒＬｅｅ基因紧密连锁。
RAPD analysis of near isogenic lines of the yellow rust resistance gene YrLee was done with 380 10 mer primers. The additional DNA fragments were amplified in the resistant near isogenic line YrLee/6 Taichung 29F 3 and the gene donor parent “Lee” by two primers, but the fragments were not amplified in the recurrent parent “Taichung 29”. The additional DNA fragment amplified by the primer OPR 7 was about 465bp, and the fragment by OPR 9 was about 667bp. The genetic linkage of the additional DNA fragment by the primer OPR 7 with YrLee was tested on a segregating F 2 population (189 plants) derived from a cross between the gene donor parent “Lee” and susceptible cultivar “Mingxian 169”. The result showed that the additional DNA fragment was closely linked to the YrLee gene.
出处
《高技术通讯》
EI
CAS
CSCD
1998年第12期11-14,共4页
Chinese High Technology Letters
基金
863计划资助
关键词
分子标记
抗病基因
小麦
RAPD
抗条锈病基因
Molecular markers, Resistance gene, Wheat, Puccinia striiformis, Yellow rust
