摘要
选用薯蓣皂苷元为对照品,以高氯酸显色,分光光度法测定胡芦巴总皂苷含量。薯蓣皂苷元在410nm处有最大吸收,在0.0163—0.0357mg.mL-1范围内吸光度与薯蓣皂苷元浓度呈良好的线性关系(r=0.9996),方法的平均回收率为98.0%(n=9),RSD为0.6%。该方法简便、准确、稳定,是一种快速测定胡芦巴总皂苷的有效方法。
The content of total saponins in triganeUa joenumgraecum L. was determined by spectrophotometry at 410nm with diosgenin as control article and colorating with perchloric acid. The calibration curve was linear in the range of 0. 0163--0. 0357mg · mL^-1 with the correlation coefficient of 0. 9996. The average recovery of diosgenin was 98. 0%（RSD= 0. 6% , n = 9）. The method is simple, accurate, sensitive and reproducible. It can be applied to the quality control of triganella joenumgraecum L.
出处
《光谱实验室》
CAS
CSCD
北大核心
2010年第2期580-582,共3页
Chinese Journal of Spectroscopy Laboratory
基金
贵州省中小企业局平台建设项目黔中小发[2008]76号
贵州省发展和改革委员会黔科合带帽字[2008]5005号
关键词
胡芦巴
总皂苷
薯蓣皂苷元
分光光度法
Triganella Joenumgraecum L.
Total Saponins
Diosgenin
Spectrophotometry
