摘要
从山东某发病肉鸽的体内分离到一株新城疫病毒(Newcastle disease virus,NDV),命名为Pigeon-Shandong-JN-08(简写JN08),对其融合蛋白(fusion,F)进行序列和分子特性的分析,以了解肉鸽NDV的遗传起源和分子特性。JN08经病毒蚀斑克隆纯化后动物回归试验可产生典型的NDV病变。研究结果发现,F蛋白多肽裂解位点的序列为112RRQKRF117,符合鸽源NDV强毒氨基酸基序。F基因分型及同源性比较显示:JN08与绝大多数鸽源毒株同属于基因Ⅵ型;其F基因与基因Ⅱ型毒株LaSota,B1,Bingham,TEX-48氨基酸同源性为89.9%,89.7%,90.4%,89.7%,与基因Ⅰ型毒株Ulster67的氨基酸同源性为91.3%,与基因Ⅲ型传统强毒F48E9的氨基酸同源性为92.8%;与国内鸽分离株P1-03,CH98-98,JS1-06,PB01-96和GZ-07的氨基酸同源性分别为91.3%,94.6%,94.8%,95.1%,94.0%;与国外鸽分离株Capital3-97,Tigre6-99,1166-02,IT227-97,2736-00,NY-84,TX3503-04,248VB-98的同源性分别为96.2%,93.8%,98.0%,96.8%,97.1%,96.8%,96.8%,98.4%。从F基因遗传进化树上发现:JN08与国外分离株的遗传距离较近,处于同一基因亚型上,而与国内分离株的遗传距离较远。
A Newcastle Disease Virus isolate named JN08 was collected from pigeon in Shandong. F gene of the isolate was cloned and sequenced to study its molecular characterization and hereditary variation. JN08 purified by plaque assay could form typical cytopathic effect. The deduced amino acid sequence of F gene showed that the virus shared a velogenic motif ^112RRQKRF^117 at the cleavage site. Phylogenetic analysis showed that JN08 and most of the viruses derived from pigeon belonged to genotype VI. For F gene of the isolate JN08,the homologies of the deduced amino acid sequences were 89.9%,89.7%,90.4%,89.7%,91.3% and 92.8% respectively,compared with strains of LaSota（Ⅱ）,B1（Ⅱ）,Bingham（Ⅱ）,TEX-48（Ⅱ）,Ulster67（I）and F48E9（Ⅲ）;the homologies of the deduced amino acid sequences were 91.3%,94.6%,94.8%,95.1% and 94.0% for isolates of P1-03,CH98-98,JS1-06,PB01-96 and GZ-07 from host pigeon in China; the homologies of the deduced amino acid sequences were 96.2%,93.8%,98.0%,96.8%,97.1%,96.8%,96.8% and 98.4% respectively for pigeon-origin isolates of Capital3-97,Tigre6-99,1166-02,IT227-97,2736-00,NY-84,TX3503-04 and 248VB-98 overseas. There was a closer genetic relationship between JN08 isolate and foreign epidemic strains that all belonged to the same gene subtype than host pigeon strains in China.
出处
《浙江农业学报》
CSCD
北大核心
2010年第1期20-24,共5页
Acta Agriculturae Zhejiangensis
基金
山东省农业科学院创新基金(2007YCX017
2006YCX024)
关键词
鸽
新城疫病毒
F基因
分子特性
pigeon
Newcastle disease virus
F gene
molecular characterization
