摘要
目的建立测定保健食品中大黄酸、芦荟大黄素、1,8-二羟基蒽醌、大黄素、大黄酚、大黄素甲醚含量的高效液相色谱法。方法使用Symmetry@C18(250mm×4.6mm,5μm)为色谱柱,以甲醇+0.1%磷酸水溶液(80+20)为流动相,检测波长为254nm。结果大黄酸、芦荟大黄素、1,8-二羟基蒽醌、大黄素、大黄酚、大黄素甲醚在0.014～0.280、0.014～0.280、0.0126～0.0252、0.0156～0.104、0.025～0.500和0.025～0.500μg/ml浓度范围内具有良好线性关系;平均加标回收率分别为91.5%、90.2%、85.3%、105.3%、87.7%和102.1%;相对标准偏差分别为6.21%、5.82%、7.11%、8.50%、7.35%和9.42%。结论该方法简便、准确,有良好的重现性,技术参数指标符合食品理化分析的要求,可用于蒽醌类保健食品的质量控制。
Objective To establish a HPLC method for the determination of rhein, aloeemodin, 1, 8- dihydroxyanthraquinone, emodin, chrysophanol and physcion in health food. Method A HPLC with Symmetry@ C18 column （250mm×4.6mm, 5 μm）, amixtureofCH3OH＋0.1% H3PO4 （80＋20） as mobile phase andaUV detector at 254 nm was used. Results The linearity for rhein, aloeemodin, 1, 8-dihydroxyanthraquinone, emodin, chrysophanol, physeion was good in the ranges of 0. 014-0. 280, 0. 014-0. 280, 0. 012 6-0. 025 2, 0. 015 6-0. 104, 0. 025-0. 500 and 0. 025-0. 500 μg/ml respectively; the recovery of those was 91.5% , 90. 2% , 85.3% , 105.3% , 87.7% and 102. 1% respectively; and the RSD of those was 6. 21% , 5.82% , 7.11% , 8.50% , 7.35% and 9.42% respectively. Conclusion The method is simple, accurate and reproducible, and could be used for the quality control of rhubarb anthraquinones in health food.
出处
《中国食品卫生杂志》
北大核心
2010年第1期27-30,共4页
Chinese Journal of Food Hygiene
