摘要
目的检测胸腺鳞癌微卫星不稳定（MSI）和杂合性缺失（LOH）发生频率，以探讨胸腺鳞癌MSI现象的合适微卫星（MS）位点。方法选择5个微卫星多态性标记，从石蜡包埋的存档标本中选取9例肿瘤组织和其对应的自身正常组织，提取DNA后用PCR扩增，6％聚丙稀酰胺凝胶电泳，银染显色后进行MSI和LOH分析。结果9例胸腺鳞癌均出现MSI或LOH。在所检5个位点中D6S1708、TP53、DM、D11S988和D8S136微卫星不平衡发生率分别为66．7％（6／9）、33．3％（3／9）、33．3％（3／9）、33．3％（3／9）和0％（0／9）。D6S1708遗传学改变多为LOH（5／6），D11S988位点仅见于LOH。结论D6S1708、TP53、DM和D11S988可以作为研究胸腺鳞癌微卫星的位点；微卫星不平衡可能在胸腺鳞癌的发生中起一定作用，其与胸腺鳞癌临床病理特点的关系尚需进一步探讨。
Objective To investigate the frequency of microsatellite instability （MSI） and loss of heterozygosity （LOH） and select sensitive loci for studying microsatellite DNA imbalance in thymic squamous cell carcinoma. Methods 5 microsatellite polymorphism markers and extrated DNA were selected from 9 specmiens of paired thymic squamous cell carcinoma/normal tissues. MSI and LOH in the specmiens of thymic carcinoma and relevant pericancerou tissues were detected by polymerase chain reaction （PCR） followed by 6% polyacrylamide gel electrophoresis（PAGE） with silver staining. Results MSI or LOH was detected in 9 thymic carcinoma tissues. The frequency of MSI or LOH was 66.7% （6/9） at loci of D6S1708, 33.3% （3/9） at TP53, 33.3% （3/9）at DM, 33.3% （3/9）at DllS988 and 0% （0/9）at D8S136, LOH at D6S1708 （5/6） was a common genetic alteration. D11 S988 had only LOH alteration. Conclusion D6S1708, TP53, DM, and D11 S988 are sensitive loci for studying microsatellite DNA imbalance in thymic squamous cell carcinoma. Microsatellite DNA imbalance may play a certain role in occurrence and development of thymic squamous cell carcinoma, and the relationship between MSI or LOH. The linicopathological features of thymic squamous cell carcinoma needs further investigation.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
2010年第1期45-48,共4页
Chinese Journal of Thoracic and Cardiovascular Surgery
关键词
胸腺肿瘤
癌
鳞状细胞
多态性
单核苷酸
微卫星不稳定
杂合子丢失
Thymus neoplasms Carcinoma squamous cell Polymorphism, single nueleotide microsatellite instability Loss of heterozygosity
