摘要
目的观测匹维溴胺对腹泻型肠易激综合征(diarrhea-prodominant irritable bowel syndrome,D-IBS)模型大鼠结肠组织P物质(SP)含量及表达变化影响,探讨匹维溴胺治疗D-IBS机制。方法采用应激与束缚相结合的方法复制D-IBS动物模型后,应用ELISA、免疫组化方法检测结肠组织SP含量及表达。结果与正常组比较,模型组大鼠结肠组织SP含量及其表达明显增高;与模型组比较,匹维溴胺组大鼠结肠组织SP含量及其表达明显降低。结论匹维溴胺治疗D-IBS模型大鼠的疗效与SP含量及其表达下调有关。
Objective To observe the effects of the Pinaverium bromide on substance P （SP） content of colon tissue in rats with diarrhea-predominant irritable bowel syndrome （D-IBS） in order to explore mechanism of action the Pinaverium bromide on D-IBS. Methods D-IBS animal modal was established by stress combined with constraint, detect SP content and expression by immunohisto- chemistry and ELISA method in colon tissue. Results Model rats compared with normal group SP were significantly elevated in colon tissue, Pinaverium bromide group compared with model group, SP were significantly lower in colon tissue. Conclusions Pinaverium bromide acts to treat D-IBS maybe connected with reducing SP in the colon tissue.
出处
《辽宁医学院学报》
CAS
2010年第1期13-15,94,共4页
Journal of Liaoning Medical University (LNMU) Bimonthly
基金
国家重点基础研究发展计划(973计划)资助项目
编号:2007CB512702
关键词
肠易激综合征
腹泻型
匹维溴胺
P物质
irritable bowel syndrome
diarrhea-predominant
pinaverium bromide
substance P
