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Molecular Characterization of Nonstructural Protein NS38 of Grass Carp Reovirus 被引量:5

Molecular Characterization of Nonstructural Protein NS38 of Grass Carp Reovirus
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摘要 Viral nonstructural proteins in both enveloped and non-enveloped viruses play important roles in viral replication. Protein NS38 of Grass carp reovirus (GCRV), has been deduced to be a non-structural protein, and, consistent with other reoviruses, is considered to cooperate with the NS80 protein in viral particle assembly. To investigate the molecular basis of the role of NS38, a complete protein was expressed in E.coli for the first time. It was found that there is a better expression of NS38 induced with IPTG at 28 ℃ rather than 37 ℃. In addition, the antiserum of NS38 prepared with purified fusion protein and injected into rabbit could be used for detecting NS38 protein expression in GCRV infected cell lysate, while there is not any reaction crossed with purified virus particle, confirming NS38 is not a component of the viral structural protein. The result reported in this study will provide evidence for further viral protein-protein and protein-RNA interaction in dsRNA viruses replication. Viral nonstructural proteins in both enveloped and non-enveloped viruses play important roles in viral replication. Protein NS38 of Grass carp reovirus (GCRV), has been deduced to be a non-structural protein, and, consistent with other reoviruses, is considered to cooperate with the NS80 protein in viral particle assembly. To investigate the molecular basis of the role ofNS38, a complete protein was expressed in E.coli for the first time. It was found that there is a better expression of NS38 induced with IPTG at 28℃ rather than 37℃. In addition, the antiserum of NS38 prepared with purified fusion protein and injected into rabbit could be used for detecting NS38 protein expression in GCRV infected cell lysate, while there is not any reaction crossed with purified virus particle, confirming NS38 is not a component of the viral structural protein. The result reported in this study will provide evidence for further viral protein-protein and protein-RNA interaction in dsRNA viruses replication
出处 《Virologica Sinica》 SCIE CAS CSCD 2010年第2期123-129,共7页 中国病毒学(英文版)
基金 National Basic Research Program (973) of China ( 2009CB118701) National Natural Scientific Foundation of China (30871940, 30671615)
关键词 Grass carp reovirus (GCRV) Nonstructural protein NS38 Recombinant expression 草鱼呼肠孤病毒 非结构蛋白 分子特性 蛋白质分子 RNA病毒 病毒复制 病毒蛋白 病毒粒子
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