摘要
目的研究人源阳离子抗菌肽(hCAP)-18/LL-37全长肽真核表达产物对树突状细胞分化成熟的影响。方法将已构建的hCAP-18/LL-37全长肽真核表达载体pcDNA4/Myc-His-hCAP-18转染Hela细胞株,在转染后48 h收获培养上清;人外周血单个核细胞分离,体外定向诱导分化成树突状细胞(DC);将转染后收获的培养上清与体外培养的DC共同孵育,48 h收获DC,经流式细胞仪检测DC表面分子CD83、CD86、CD40及HLA-DR的表达。结果FCM结果显示:pcDNA4/Myc-His-hCAP-18转染Hela细胞培养上清与pcDNA4/Myc-His空载体转染细胞培养上清相比较,均可上调DC表面分子CD86、CD40及HLA-DR的表达。结论构建的hCAP-18/LL-37全长肽真核表达载体pcDNA4/Myc-His-hCAP-18转染Hela细胞株培养上清液均可上调DC表面分子CD86、CD40及HLA-DR的表达,促进DC的分化成熟。
Objective Objective To study The effects of eukaryotic expression products of the whole range peptide of hCAP-18/ LL-37 on differentiation and maturation of DC. Methods pcDNA4/Myc-His-hCAP-18 for the whole peptide was transfected into Hela cell line. After the cells were cultured for 48 h the supernatant was collected. We prepared human peripheral blood monouclear cells and induce them into DC in vitro. Then we add the supematant collected from the transfected cells and continued culturing the DC for 48 h. By FCM we detected the surface molecules on DC which are related with the differentiation and maturation of DC such as CD83, CD86, CIMO and HLA-DR. Results We detected the mature peptide expression in＇the Hela cell transfected by pcDNA4/Myc-His- hCAP-18. FCM indicated that CD86 ,CD40及. HLA-DR on the surface of DC which were stimulated by the pcDNA4/Myc-His-hCAP- 18 supernatant are more expressed than them on the surface of DC which were stimulated by the supernatant produced by pcDNA4/ Myc-His naked vector. Conclusion The whole range peptide of hCAP-18/LL-37 that we constructed and expressed can increase the expression of the DC surface molecules which are related with the differentiation and maturation of DC and promote differentiation and maturation of DC.
出处
《中国实验诊断学》
北大核心
2010年第3期346-348,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科学技术厅发展计划项目(200705357)
