摘要
目的建立RP-HPLC法同时测定桂枝茯苓胶囊中没食子酸、白芍苷和芍药苷含量。方法色谱柱：大连中汇达C18柱（4．6mm×250mm，5μm），流动相：乙腈-体积分数为0．1％的磷酸溶液，梯度洗脱，流速：1．0mL·min^-1，检测波长：230nm，柱温：40oC。结果没食子酸、白芍苷和芍药苷的线性分别为0．69～6．87mg·L^-1（，=0．9996）、0．88—8．80mg·L^-1（r=0．9996）和2．00—20．00mg·L^-1（r=0．9995）。平均回收率：没食子酸为99．7％（RSD=1．0％，n=9）、自芍苷为101．5％（RSD=1．5％，n=9）、芍药苷为100．0％（RSD=0．7％，月=9）。结论此方法可为桂枝茯苓胶囊的质量控制提供方法。
Objective To establish an RP-HPLC method for the determination of gallic acid, albiflorin, paeoniflorin in Guizhi Fuling capsules（ traditional Chinese medicines）. Methods The separation was carried out on a Kromasil C18 （4. 6 mm ×200 mm,5μm）column with a mobile phase of acetonitrile -0.1% （ V： V）phosphoric acid solution under gradient elution. The flow rate was 1.0 mL·min^-1 and the column temperature was 40℃. The detection wavelength was set at 230 nm. Results The calibration curve was linear within the range of 0.69- 6. 87 mg·L^-1（ r = 0. 999 6） ,0. 88- 8. 80 mg·L^-1 （ r = 0. 999 6 ） and 2.00 - 20.00 mg·L^-1 （ r = 0. 999 5 ） for gallic acid, albiflorin and paeoniflorin, respectively. The average recoveries were 99.7 % （ RSD = 1.0%, n = 9）, 101.5 % （ RSD = 1.5 % , n = 9） and 100. 0% （ RSD = 0. 7 %, n = 9 ） for gallic acid, albiflorin and paeoniflorin, respectively. Conclusions This method is convenient, accurate and reproducible for the determination of gallic acid, albiflorin and paeoniflorin in Guizhi Fuling capsules.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2010年第3期216-219,共4页
Journal of Shenyang Pharmaceutical University
