摘要
利用PCR-RFLP法对大豆根腐病的4种致病菌和3种非致病菌代表菌株进行分子判别研究。结果显示，7个菌株DNA进行PCR扩增得到的rDNA-ITS序列片段长度为600900bp，除立枯丝核菌、瓜果腐霉和毛霉菌代表菌株DNA扩增出的rDNA-ITS片段长度之间没有显著差异外，其他菌株DNA扩增出的rDNA-ITS片段长度差异显著。因此各菌株通过PCR-RFLP法扩增出的rDNA—ITS序列片段之间的大小差异可将侵染大豆的各根腐病致病菌和非致病菌初步区分开。而利用A／uI内切酶对各菌株的PCR产物进行酶切，依据酶切片段之间的大小差异，可进一步将7种菌株区分开，因此利用rDNA-ITS序列分析方法对大豆根腐病这种复合侵染病害的病原菌进行分子判别具有可行性，同时也是建立作物根部复合侵染病害病原种类的分子检测的一种重要方法。
The present study investigated the molecular identificiation of the 4 pathgenic isolates and 3 unpathgenie isolates of the soybean root rot by the PCR-RFLP method in this paper. The results showed that the rDNA-ITS fragements from the seven isolates was bettween the 600-900 bp by the PCR and there weren＇t significant differences among the rDNA-ITS fragements of the Rhizoctonia,Pythium aphani dermaturn and Mucor,in the other rDNA-ITS fragements exited significant differences. Therefore, we could differentiate partly isolates by the PCR of the seven isolates. Furthermore, we could further differentiate seven isolates ,base on the fragements size of the PCR product from the seven isolates digested by Alu I were different. Therefore, this molecular identification technoloy is feasible to identify the complex invasive disease of the soybean. At the same time, it is also an important method to set up the molecular detection technology of the complex invasive disease of the corn root rot.
出处
《新疆农业大学学报》
CAS
北大核心
2010年第2期151-154,共4页
Journal of Xinjiang Agricultural University
基金
新疆维吾尔自治区科学研究和技术开发项目(ZOO631104)
