血管平滑肌细胞中ERK通路与TGF-β1/Smad通路的相互作用被引量：1

Interaction between TGF-β_1/Smad pathway and ERK pathway in vascular smooth muscle cells

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摘要目的:探讨血管平滑肌细胞(VSMC)中TGF-β/Smad与ERK信号转导通路是否存在相互调节关系。方法:原代培养的大鼠胸主动脉平滑肌细胞,分四组:①对照组,②TGF-β1组,③ERK阻断剂(PD98059)组和④TGF-β1+ERK阻断剂(PD98059)组。分别用Western blot法检测VSMC内Smad2/3、ERK1/2蛋白表达及磷酸化Smad2/3、磷酸化ERK1/2蛋白含量,RT-PCR方法测VSMC中Smad2、Smad3mRNA的表达。结果:①与对照组相比,TGF-β1组P-Smad2/3、P-ERK1/2蛋白含量增多(P<0.05),ERK阻断剂组P-Smad2/3、P-ERK1/2蛋白含量减少(P<0.05),TGF-β1+ERK阻断剂组P-Smad2/3、P-ERK1/2蛋白含量无差异;与TGF-β1组相比,TGF-β1+ERK阻断剂组P-Smad2/3、P-ERK1/2蛋白含量减少(P<0.05)。各组间Smad2/3、ERK1/2蛋白表达无差异。②各组的Smad2、Smad3mRNA表达无差异。结论:TGF-β1诱导的Smad2/3蛋白磷酸化依赖ERK通路激活,但ERK通路对Smad2/3蛋白和mRNA表达水平无影响。 Objective：To investigate if the interaction between TGF-β1/Smad pathway and ERK pathway in vascular smooth muscle cells exists.Methods：The rat arota was removed.The primary VSMC were isolated and cultured in vitro,then the VSMC were divided into four groups：①control group,②TGF-β1 group,③ERK blocking agent group,④TGF-β1＋ ERK blocking agent group.The expression of Smad2/3,ERK1/2 proteins,the content of phosphorylated ERK1/2 and Smad2/3 proteins were detected by Western blot,and the expression of Smad2/3 mRNA was detected by reverse transcription-polymerase chain reaction（RT-PCR）.Results：①In contrast to control group,the content of phosphorylated Smad2/3 and phosphorylated ERK1/2 proteins in TGF-β1 group was increased（P〈0.05）,that in ERK blocking agent group was decreased（P〈0.05）.There was no difference between control group and TGF-β1＋ERK blocking agent group.Compared with TGF-β1 group,the contents of phosphorylated Smad2/3 and phosphorylated ERK1/2 proteins in TGF-β1＋ERK blocking agent group was decreased（P〈0.05）.There was no difference in the expression of Smad2/3 and ERK1/2 proteins among different groups.②There were no differences in expression of Smad2 and Smad3 mRNA among different groups.Conclusion：①TGF-β1 can induce Smad2/3 proteins to be phosphorylated dependent on the activated ERK pathway.②ERK pathway does not effect the expression of Smad2/3 at the level of protein and mRNA.

作者钟华何芳胡清华王振焕邓峰美孙志萍李增春

机构地区新疆地方病与民族高发病教育部重点实验室石河子大学医学院病理生理教研室石河子大学医学院医学机能实验中心华中科技大学同济医学院病理生理学系

出处《中国应用生理学杂志》 CAS CSCD 北大核心 2010年第1期15-18,共4页 Chinese Journal of Applied Physiology

基金国家自然科学基金资助(30760077)

关键词 ERK 血管平滑肌细胞 TGF-β_1 ERK VSMC TGF-β1

分类号 R363.2 [医药卫生—病理学]

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