摘要
目的:探讨对舒心方缺氧环境下人脐静脉内皮细胞增殖和细胞周期的影响。方法:建立人脐静脉内皮细胞缺氧模型,采用MTT法测定细胞增殖,流式细胞术分析细胞周期。结果:舒心方能够明显促进缺氧环境下内皮细胞的增殖,增加细胞周期中S期细胞的数目。结论:舒心方对缺氧环境下内皮细胞增殖和DNA合成有促进作用,提示舒心方在缺血心肌新生血管生成过程中可能具有一定作用。
Objective：To investigate effects of shuxin formula on the proliferation of cultured human umbilical vein endothelial cells（HUVECs） injured by hypoxia.Methods：HUVECs were cultured and hypoxic models were established,cell proliferation was determined with MTT methods,cell cycle was analyzed with cytometry.Results：shuxin formula could notably promote the growth of endothelial cells in hypoxic condition,and increase the cell population in S phase.Conclusion：shuxin formula has effect in promoting proliferation of endothelial cell and its DNA synthesis,it is suggested that shuxin formula could exert some effect on the process of angiogenesis in ischemic myocardium.
出处
《中华中医药学刊》
CAS
2010年第3期529-530,共2页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科研计划资助课题(03B017)
关键词
舒心方
内皮细胞
缺氧
细胞增殖
细胞周期
shuxin formula
endothelial cells
hypoxia
cell proliferation
cell cycle
