摘要
建立了贴片中标示成分芍药苷含量的HPLC分析方法和贴片中各种残留溶剂含量的GC分析方法。样品经甲醇提取,离心分离后,以乙腈-水(体积比16:84,HAc0.4%)为流动相,选择ODS-2 Hypersil C1(84.6mm×150mm,5μm)色谱柱,测定芍药苷含量。贴片基质经处理,以GC内标法测定残留溶剂含量。结果表明HPLC法的精密度、重现性和回收率良好;GC分析测得乙酸乙酯、异丙醇、乙醇三种残留溶剂,贴片中残留溶剂总量为2.1×10-4。
A HPLC and GC method was established to detect content ofpaeoniflorin and residual solvents. After the sample was extracted with methanol and separated by centrifuger ,paeoniflorin content was detected with ODS-2 Hypersil Ct8 （4.6 mm × 150 mm, 5 V m）column, using acetonitrilewater （v/v 16：84, HAc 0.4% ）as mobile phase. After the matrix was dissolved by aether and separated by centrifuger, content of residual solvents was detected by using GC internal standard method. Results show that the RSD,reproducibility and recoveries of the method are good;Ethyl acetate,isopropanol and ethanol have been detected by GC, with the totall content of residual solvents 2.1 × 104.
出处
《当代化工》
CAS
2010年第1期102-105,共4页
Contemporary Chemical Industry
关键词
乳癖消
压敏胶贴片
芍药苷
残留溶剂
含量测定
Rupixiao
PSA Patch
Paeoniflorin
Residual Solvents
Determination of Content
