摘要
目的探讨骨桥蛋白在肝癌转移中的作用以及Ｈ－ｒａｓ基因和骨桥蛋白表达的相互关系。方法对２例正常肝脏组织、１６例肝癌患者癌及癌周组织进行ｍＲＮＡ提取，采用ＲＴ－ＰＣＲ半定量法研究了肝癌及癌周组织中骨桥蛋白以及Ｈ－ｒａｓ的表达。结果２例正常肝脏组织中骨桥蛋白和Ｈ－ｒａｓ均无表达，１６例肝癌组织中骨桥蛋白及Ｈ－ｒａｓ表达均高于相邻的癌周组织，经ｔ检验，Ｐ均＜００５。在１３例骨桥蛋白高表达的病例中，癌周浸润率为６９２％（９／１３），癌转移率为３０８％（４／１３）。肝癌及癌周组织中骨桥蛋白和Ｈ－ｒａｓ表达值的直线相关系数分别为０６０５和０４８６，Ｐ均＜００５。结论肝癌组织中骨桥蛋白的表达可能和肝癌细胞的发生有关，骨桥蛋白高表达和肝癌癌周浸润以及转移密切相关，肝癌组织中骨桥蛋白的表达和Ｈ－ｒａｓ的表达呈直线正相关。
Objective To study the role of osteopontin(OPN) in metastasis of hepatocellular carcinoma and the relations between OPN and H-ras. Methods mRNA was extracted from 2 samples of normal liver tissues and from tumors and tumor-surrounding tissues in 16 cases of hepatocellular carcinoma. Semi-quantity RT-PCR was used to research on the expressions of OPN and H-ras in tumors and tumor-surrounding tissues. Results There were no expressions of OPN and H-ras in 2 samples of normal liver tissues. In 16 cases, the expressions of OPN and H-ras in tumors were all higher than those in tumor-surrounding tissues. P values were lower than 0 05 in T Test. In 13 cases of higher expression of OPN, the invasive rate and metastatic rate of tumors were 69 2%(9/13) and 30 8%(4/13) respectively. The lineal correlation coefficient between OPN and H-ras in tumors and tumor-surrounding tissues were 0 605 and 0 486 respectively. Conclusion The expression of OPN was closely related to carcinogenesis, invasiveness and metastasis of hepatocellular carcinioma, and the expression of OPN and H-ras in tumors and tumor-surrounding tissues were positively lineal correlated.
出处
《中华肝脏病杂志》
CAS
CSCD
1998年第4期202-210,共9页
Chinese Journal of Hepatology
基金
上海市领先学科项目(94-Ⅲ-2)资助
关键词
肝癌
骨桥蛋白
肿瘤转移
Hepatocellular carcinoma Osteopontin Metastasis
