摘要
为探讨全反式维甲酸在人卵巢癌细胞生长和增殖中的作用机制,选用人卵巢癌细胞株HO8910在体外进行培养,培养时添加不同浓度的全反式维甲酸(all-trans retinoic acid,ATRA),作用24h、48h、72h后,采用MTT比色法测定细胞生长抑制率;采用流式细胞术(flow cytometry,FCM)进行细胞周期分析;采用逆转录多聚酶链反应(reverse transcription polymerasechain reaction,RT-PCR)检测维甲酸α受体(retinoic acid receptorα,RARα)mRNA的表达。结果是不同浓度的全反式维甲酸对HO8910细胞生长均起到抑制作用(P<0.05),并呈浓度和时间依赖关系;能够增加G1期细胞比例,同时降低S、G2/M期细胞比例(P<0.05),细胞增殖指数PI?(P<0.05)降低;能够上调维甲酸α受体mRNA的表达(P<0.05),从而抑制卵巢癌细胞增殖。说明全反式维甲酸对卵巢癌细胞HO8910的增殖具有显著的抑制作用,其机制可能是通过上调维甲酸α受体mRNA的表达来实现的。
To study the effect of all-trans retinoic acid （ATRA） in the proliferation of human ovarian cancer cells, HO8910 human ovarian cancer cell lines were cultured in vitro and treated with different concentrations of all-trans retinoic acid for 1,2 or 3 days. Cell proliferation was evaluated by MTT assay. Cell cycle was analayzed by flow cytometry （FCM）. The expression of retinoic acid receptor α mRNA was detected by reverse transcription- polymerase chain reaction （RT-PCR）. It is resulted that different concentrations of all-trans retinoicacid could inhibit the growth of HO8910 cells （P 〈 0.05 ） in a time and dose-dependent manner. All-trans retinoic acid increases the rates of G1 and decreases the rates of S and G2/M （P 〈 0.05 ）, and therefore, it decreases cells proliferation index （ PI^＊ ） （ P 〈 0.05 ）. It could up-regulate the expression of retinoic acid receptor α mRNA（P 〈 0.05 ）, then inhibit proliferation of HO8910 cells. It is conclused that all-trans retinoic acid could inhibit the proliferation of human ovarian cancer cells HO8910, the molecular basis may be associated with the up-regulated mRNA of retinoic acid receptor α.
出处
《科学技术与工程》
2010年第8期1844-1847,1852,共5页
Science Technology and Engineering
关键词
全反式维甲酸
维甲酸α受体
细胞周期
all-trans retinoic acid （ATRA） retinoic acid receptor α cell cycle
