摘要
本文对森原等人合成人胰岛素的方法进行了改进。用羧肽酶A和胰酶成功地实施了人胰岛素的半合成。精品的总收率为70％,高于1986年森原等报道的水平(50％)。 苏氨酸叔丁酯是半合成人胰岛素的主要原料之一。我们改进了它的合成方法。
Trypsin was used in catalysing the coupling between desalanine-B30-insulin(DAI) and Thr(Bu)OBu, DAI was obtained by digestion of porcine insulin with carboxypeptidase A. Thr(Bu)OBu was directly synthesized without N-terminal protection, different from general way and a high yield of human insulin was obtained. The purity of the semi-synthetic human insulin was one band of electrophoresis. It can be easily crystallized. The amino acid analysis of the semi-synthetic human insulin was identical with the theoretical value. The radiation inactivation test shows the semi-synthetic human insulin has the same insulin-binding activity with the porcine insulin.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1989年第4期410-411,共2页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
胰岛素
胰酶
半合成
羧肽酶A
Insulin, Trypsin, Semi-synthesis, Carboxypeptidase A
