摘要
目的调查大肠埃希菌尿液分离株中质粒、转座子和整合子等可移动遗传元件的存在情况。方法收集宁波市第一医院2008年10月～2009年3月患者尿液标本中分离的大肠埃希菌共28株,采用聚合酶链反应(PCR)及序列分析的方法分析10种可移动遗传元件:traA、trbC、tnpA、tnpU、tnp513、tnsA、merA、intⅠ1、intⅠ2、intⅠ3。结果28株大肠埃希菌共检出3种基因:traA、trbC、intⅠ1,其余7种基因未检出;各种基因阳性率以traA最高25株(89.3%),intⅠ1次之19株(67.9%),trbC8株(28.6%);所有28株(100.0%)至少检出1种基因,各菌株基因阳性数1～3种,5株ECO同时检出3种基因,13株同时检出2种基因,10株仅检出1种基因。结论同时检测10种可移动遗传元件是国内首次报道,traA和intⅠ1基因阳性率较高,而trbC基因阳性率较低;可移动遗传元件介导各种耐药基因使受体菌表现为多药耐药,检出trbC基因为国内首次报道。
OBJECTIVE To investigate the distribution of mobile genetic elements：plasmids,transposons,and integrons in multi-drug resistant strains of Escherichia coli isolated from urine. METHODS From Oct 2008 to Mar 2009,28 multi-drug resistant strains of E. coli isolated from urine were collected from Ningbo No.1 Hospital,China. Then,10 kinds of mobile genetic elements （traA,trbC,tnpA,tnpU,tnp513,tnsA,merA,intⅠ1,intⅠ2,and intⅠ3） were analyzed by PCR and verified by DNA sequencing. RESULTS In 28 strains of E. coli,25 strains（89.3%）,19 strains（67.9%）and 7 strains（25.0%）were detected to carry traA,intⅠ1 and trbC,respectively. However,the other 7 kinds of genes couldn＇t be detected out. Furthermore,all 28 strains（100.0%）were detected to carry at least 1 kind of gene. In addition,5 strains were detected to carry 3 kinds of genes,13 strains were detected to carry 2 kinds of genes,and 10 strains were detected to carry 1 kind of gene. CONCLUSIONS It＇s the first report to detect 10 kinds of mobile genetic elements in China. The positive rate of traA and intⅠ1 carryed on 28 multi-drug resistant strains of E. coli are much higher than that of trbC. And acquired resistance genes induced by mobile genetic elements cause the recipient strains to express multi-drug resistance. Moreover,it＇s the first report that trbC is detected in China.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2010年第5期607-610,共4页
Chinese Journal of Nosocomiology
关键词
大肠埃希菌
尿液
质粒
转座子
整合子
可移动遗传元件
多药耐药
Escherichia coli
Urine
Plasmid
Transposon
Integron
Mobile genetic elements
Multi-drug resistance
