摘要
以两种不同的路线合成β－环瑚精－环氧丙氧丙基键合硅胶固定相（β＿ＣＤ－ＧＰＳ）。比较了两种方法得到的键合相的键合量。采用非水滴定法考察了影响键合反应的因素。选择了合成β＿ＣＤ－ＧＰＳ最佳反应条件，并对其色谱性能进行了考察。
β _Cyclodextrin- γ _glycidoxypropylsilyl bonded phase ( β _CD-GPS) for high performance liquid chromatography was synthesized via two different routes:(i) KH_560 reacted on the silica to produce GPS and then β _CD-GPS was obtained by the reaction of β _CD with GPS;(ii)the mixture of the reaction of β _CD with KH_560 reacted on the silica to produce β _CD-GPS.The amounts of bonded β _CD for two stationary phases were calculated according to the results of elemental analysis.The reaction conditions were optimized on the basis of the content of α _epoxy group of γ _glycidoxypropyl bonded silica.The chromatographic performance of β _CD-GPS was studied.
出处
《分析测试学报》
CAS
CSCD
1998年第5期5-8,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金
关键词
高效液相色谱
环糊精键合
固定相
硅胶
HPLC, β _CD bonded stationary phase,Optimization of bonded reaction conditions
