摘要
通过PCR方法从重组质粒pGEM-ORF5扩增得到缺失N端疏水序列的基因片段dORF5(deleting ORF5)。将dORF5克隆至原核高效表达载体pGEX-4T-1,在E.coliRosetta细胞中成功表达了重组蛋白GST-dORF5。用Western blotting鉴定表达蛋白,证明dORF5基因得到表达。本试验得到的重组蛋白,为进一步研究PRRS病毒结构蛋白的结构和功能奠定了基础。
The truncated ORF5 gene segments of PRRS which deleted hydrophobic N-terminal sequence,amplified from recombi- nant plasmids p-GEM-ORF5 and were cloned into prokaryotic expression vector pGEX-4T-1. The recombinant proteins GST - dORF5 were highly expressed in Escherichia coli Rosetta. Western blotting analysis showed the recombinant protein could react with antibody. The result provided fundamental data and materials for the further study on the structure and function of GP5 protein of PRRSV.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第3期131-134,共4页
Biotechnology Bulletin
关键词
猪繁殖与呼吸综合征
ORF5基因
原核表达
Porcine reproductive and respiratory syndrome Gene ORF5 Prokaryotic expression
