摘要
以心叶喜林芋带芽茎段作外植体，建立了完善的组织培养体系。结果表明：外植体最适取材时间是春季，尤其是4月份；芽启动萌发的最佳培养基为MS＋6BA3．0mg／L＋NAA0．1mg／L，萌发率达88．86％；是增殖培养的理想培养基MS＋6BA4．0mg／L＋NAA0．2mg／L，增殖倍数可达5．7；最适生根培养基为：1／2MS＋NAA0．1mg／L＋IBA0．2mg／L＋0．1％AC，生根率可达100％；以珍珠岩：河沙：蛭石（1：1：2）为无菌苗的移栽基质，移栽成活率达到92．5％，且在基质温度达15-20℃，室温22～25℃时无菌苗生长情况最好。
Using stem segments as explants, systematic researches were carried out to develop a plant regeneration system for Philodendron gloriosum Andre. The results showed that the best season of isolating explants was spring, especially april;The medium MS＋6 BA 3. 0 mg/L＋NAA 0. 1 mg/L was suitable for bud sprouting, in which the bud sprouting rate was 88. 86% ;The medium MS＋6-BA 4. 0 mg/L＋NAA 0. 2 mg/L was suitable for the multiplication of shoots, resulting in a multiplication rate of 5.7;The optimum culture medium for rooting was 1/2MS＋NAA 0. 1 mg/L＋IBA 0. 2 mg/L＋0. 1% AC. The transplanting medium of perlite, sand and vermiculite by 1 ： 1 ： 2 was optimal, in which the ratio of survival could reach 92. 5%, and the plants could grow normally when medium temperature and room temperature reached 15 -20 ℃ and 22-25℃ repectively.
出处
《北方园艺》
CAS
北大核心
2010年第6期165-167,共3页
Northern Horticulture
关键词
心叶喜林芋
茎段
组织培养
Philodendron gloriosum
tissue culture
stem segment with bud
