摘要
采用甲状腺细胞单层培养及标记免疫分析法,对比研究α-干扰素(IFN-α)对正常与Graves病(GD)甲状腺细胞生成cAMP的影响,结果显示:(1)基础状态下,10^(-3)、10^(-1)和10u/ml的IFN-α可刺激正常甲状腺细胞产生cAMP,刺激强度随IFN-α浓度的增大而减弱,当试验剂量达10~3u/ml时,cAMP的生成量与无刺激的对照组比较无统计学差异。此浓度区间的IFN-α对GD甲状腺细胞的cAMP的释放无显著影响。(2)IFN-α在10^(-3)～10u/ml范围内,可明显增强TSH对正常甲状腺细胞生成cAMP的刺激作用,但10~3u/ml的IFN-α则抑制TSH的刺激效应。在10^(-3)～10~3u/m的浓度范围,IFN-α能够增强TSH刺激GD甲状腺细胞释放cAMP,且GD细胞对相同浓度的IFN-α的反应强度明显大于正常甲状腺细胞。提示IFN不仅参与正常甲状腺功能的调控,而且还可能在GD的发生发展中发挥重要作用。
We studied the effect of interferon-α(IFN-α) on c-AMP production from human normal and hyperfunctioning thyrocytes with cell culture and radioimmunoassay. The results showed that: (1) At the concentration range of 10-3-10u/ml, TFN-α could stimulate basal cAMP production from normal thyrocytes. With increase of the concentration, the effect of IFN-α reduced gradually. However, TFN-α in the same range of concentrations possessed no effect on hyperfunctining thyroid cells. (2)IFN-α in the concentration of 10-3-10u/ml significantly promoted TSH-induced cAMP generation from normal thyrocytes, while IFN-α at 103u/ml exerted an inhibitory effect. In the same concentrationrange, IFN-α increased TSH-stimulated cAMP production from hyperfunctioning thyroid cells. The stimulatory effect of IFN-α on hyperfunctioning thyrocytes was much stronger than that on normal thyroid cells. The results suggest that IFN-α modulates the function of human thyroid gland. The responsiveness of normal and Graves' thyroid tissues to IFN-α is quite different.
出处
《放射免疫学杂志》
CAS
1998年第6期329-331,共3页
Journal of Radioimmanology
基金
江苏省自然科学基金
江苏省卫生厅青年基金资助课题
