摘要
通过黄酮类物质特异颜色反应和可见分光光度法,筛选产黄酮的灯盏花内生真菌,研究灯盏花产黄酮内生真菌在PDA和查氏培养基上的产黄酮能力。结果表明,28株灯盏花内生真菌中,7株菌的镁粉+浓盐酸、氯化铝、浓氨水3种颜色反应均呈阳性,能够产生黄酮。灯盏花产黄酮内生真菌的生长、菌丝体黄酮含量和产量在PDA培养基上均高于在查氏培养基上。7株灯盏花产黄酮内生真菌中,菌株ELF3-1和ERF3-1的生长较好,菌株ELF′3-1、ELF3-2和ERF3-1菌丝体黄酮含量较高,菌株ERF3-1菌丝体黄酮产量最高。
The endophytic fungi isolated from Erigeron breviscapus that could produce flavonoid were selected by the way of characteristic color reaction and visible spectrophotometry of flavonoid, their flavonoid producing capacity was studied on PDA and Czapek media, the results showed that： the reactions with Mg and concentrated hydrochloric acid, AlCl3 and concentrated ammonia liquor of 7 strains in 28 endophytic fungi strains isolated from Erigeron breviscapus were positive, that meant this 7 strains could produce flavonoid. The hypae biomass, flavonoid content and production of flavonoid producing endophytic fungi on PDA medium were greater then on Czapek medium. In the 7 flavonoid producing endophytic fungi strains, the growth of strains ELF3 - 1 and ERF3 - 1 were best, while the hypae flavonoid content of strain ELF＇3 - 1 ,ELF3 - 2 and ERF3 - 1 were great, the flavonoid production of strain ERF3 - 1 was highest.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2010年第B03期226-229,共4页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(30660040)
云南省教育厅青年科研基金项目(A3003106)
关键词
灯盏花
内生放线菌
黄酮
Erigeron breviscapus
endophytic fungi
flavonoid
