摘要
目的:探讨脐全血细胞组成特点,对脐血造血干／祖细胞进行定量测定。方法:采用自动血细胞分析仪检测血细胞成份,以流式细胞术为基础.应用双荧光标记单克隆抗体方法,测定造血于／祖细胞的含量。结果:脐血组成细胞的大多数检测指标高于成人外用血。CD34＋细胞占1.14±1.12％;CD34＋CD38-细胞占CD34＋细胞的16％。结论:脐血具有丰富的血细胞及造血于／祖细胞,有造血重建的临床应用潜能。
Object: To explore the characters of the cell composition of cord blood and the contents of hematopoietic stem/progenitor cells in cord blood. Metbods: The composition of cord blood cells was analysed by the antomatic hematology analyzer. The contents of hematopoietic stem/progenitor cells were mearsured by the Flow Cytometer (FCM) staining with monoclonal antibody labeled by double fluoresceins (FITC and PE). Results: Most of the levels of blood cells composition in cord blood were higher than adult peripheral blpod, The mean percentage of CD34+ cells was 1. 14±1. 12%;About 16% of CD34+cells were CD34+CD38- cells. Conclusion; There were plenty of blood cells and hematopoietic stem/progenitor cells in cord blood, and cord blood has potential clinical applications for hematopoietic reconstitution.
出处
《镇江医学院学报》
1998年第4期437-438,共2页
Journal of Zhenjiang Medical College
基金
江苏省教委资助!93106
江苏省卫生厅资助!H9509
