摘要
目的:建立超高效液相色谱法测定榄香烯脂质体的药物含量及包封率。方法:色谱柱为XR-ODSⅡ柱(2.2μm,75mm×3.0mm),流动相为乙腈-水(80∶20),柱温为40℃,检测波长为210nm,流速为1.0mL·min-1。结果:色谱条件下榄香烯与辅料、溶剂峰分离良好,β-榄香烯在1～100μg·mL-1范围内线性关系良好。榄香烯脂质体平均包封率为(99.8±0.4)%。结论:超高效液相色谱法可用于检测榄香烯脂质体的含量和包封率,简单、快速且准确。
Objective：To develop an UPLC method for determination of content and entrapment efficiency of elemene liposomes.Methods：The analytes was separated on a XR-ODSⅡ（2.2 μm,75 mm×3.0 mm）column with acetonitrile-water（80∶20）as the mobile phase at a flow rate of 1.0 mL·min-1.The column temperature was 40 ℃ and detection wavelength was set at 210 nm. Results：Elemene had a good linear relation in a range of 1-100 μg·mL-1.The average entrapment efficiency of elemene liposomes reached（99.8±0.4）%.Conclusion： UPLC method for determination of content and entrapment efficiency of elemene liposomes was simple, fast and accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第3期508-510,共3页
Chinese Journal of Pharmaceutical Analysis
基金
浙江省科技厅资助项目(2009C33005)
国家青年自然基金项目(20906016)
杭州市科研院所专项项目(20090331N03)
