摘要
目的:体外培养冠心病患者外周血单个核细胞来源的树突状细胞(dendritic cells,DCs),并利用基因芯片技术对冠心病患者外周血培养的DC功能基因表达谱进行研究。方法:分别配对筛选急性冠状动脉综合征患者(ACS)及冠状动脉正常的胸痛综合征患者各5例,将人外周血分离的单个核细胞加入包含rhGM-CSF(20μg/L)和rhIL-4(20μg/L)的培养基中培养,使其分化为DCs。再用基因芯片技术检测DC特异性功能蛋白基因表达的变化。结果:ACS组功能蛋白表达基因中有5个基因呈明显上升,分别是G1P2、G1P3、IFIT4、IL-1β和MX1,这些都是高度相关的干扰素诱导蛋白基因。有17个基因呈明显下降表达,分别是ACPP、AIM2、ATM、CCR1、CCR5、CD1C、FCGR3A、IFI16、IL-16、IL-18、LY75、MAP4K3、TAP1、TAP2、TLR1、TNFRSF6和VCL等,分别属于抗原识别受体、细胞趋化因子受体、细胞因子和细胞内信号传导系统。结论:冠心病患者外周血单个核细胞来源的DC不但在形态和表型上存在明显差异,而且在功能蛋白表达方面也存在显著变化。
Objective：To study the genes expression profile of monocyte derived dendritic cells from patients with coronary heart disease by Gene array. Method：Five matched-pairs were screened from patients with acute coronary syndrome and people with chest pain but normal coronary arteries. DCs derived from peripheral blood monocytes （ PBMCs） were isolated and cultured in vitro with rhGM-CSF （20 ng/ml）and rh IL-4 （20 ng/ml）. the DCs＇ expression profile of cytokine and signal transduction genes were examined by the gene array. Results：Detection by Gene array showed that 5 functional expression genes,including G1P2,G1P3,IFIT4,IL-1β and MX1,increased obviously in ACS cases. All they are the highly correlated interferon-induced protein genes. 17 genes,including ACPP,AIM2,ATM,CCR1,CCR5,CD1C,FCGR3A,FI16,IL-16,IL-18,LY75,MAP4K3,TAP1,TAP2,TLR1,TNFRSF6 and VCL,expression levels decreased obviously. They belong to antigen recognition receptors,cell chemokine receptors,cytokines and intracellular signal transducting system,respectively. Conclusion：The results suggested that the Genes Expression Profile of DCs from patients with ACS were different from people with CPS. This indicated that DCs may play the important role in the form of atherosclerosis.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2010年第1期7-10,共4页
Journal of Clinical Cardiology
