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14-3-3tau蛋白通过ERK信号通路调控BeWo细胞的侵袭功能 被引量:1

14-3-3 tau regulates invasiveness of BeWo cells via ERK1/2 pathway
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摘要 目的:探讨14-3-3tau蛋白对滋养细胞侵袭功能的影响及可能机制。方法:免疫组化法检测人早孕期绒毛、蜕膜以及人绒癌滋养细胞株BeWo中14-3-3tau的表达。RNA干扰方法下调BeWo细胞14-3-3tau的表达,RT-PCR和Western blotting方法检测E钙黏附素及转录因子snail的表达变化,Transwell小室方法检测侵袭细胞数目的变化。用U0126特异性阻断胞外信号调节蛋白激酶1/2(ERK1/2)信号通路,观察下调14-3-3tau对BeWo细胞侵袭能力的变化。结果:(1)14-3-3tau在早孕期绒毛外细胞滋养细胞和侵袭入蜕膜组织的游离型滋养细胞以及BeWo细胞中均有表达。(2)下调BeWo细胞14-3-3tau表达后,E钙黏附素表达下降,Snail表达增加,穿过Transwell小室的细胞数增加(P<0.05)。(3)U0126阻断14-3-3tau下调引起的BeWo细胞侵袭增加(P<0.05)。结论:14-3-3tau通过ERK1/2信号通路抑制滋养细胞的侵袭能力。 AIM: To investigate the potential role of 14-3-3 tau in trophoblast cells on invasiveness. METHODS: 14-3-3 tau expression was detected in first-trimester villi, deciduas and human trophoblastic cell line (BeWo) by immunohistochemistry. Small interference RNA (siRNA) targeting 14-3-3 tau was transfected into BeWo cells. The effects of down-regulated 14-3-3 tau on invasion of human trophoblasts cell line BeWo were examined by matrigel invasion assay, and the transcription, translation of E-cadherin and snail were estimated by RT-PCR or Western blotting. U0126 was used to detect the extracellular-signal related kinase 1/2 (ERK1/2) function on down-regulation of 14-3-3 tau induced cell invasion. RESULTS: 14-3-3 tau was detected in the invasive trophoblastic cells in the first trimester villi and that invaded to the deciduas. BeWo cells also expressed 14-3-3 tau. Down-regulation of 14-3-3 tau increased the invasive cell-number of BeWo, as well as the expression of snail, and inhibited E-cadherin. U0126 inhibited the enhanced invasiveness in these cells induced by the down-regulation of 14-3-3 tau. CONCLUSION: 14-3-3 tau may regulate the invasiveness of human trophoblastic cells through ERK1/2 signaling pathway.
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2010年第3期563-567,共5页 Chinese Journal of Pathophysiology
基金 国家青年科学基金资助项目(No.30600657) 国家自然科学基金资助项目(No.30872777) 高等学校博士学科点专项科研基金资助项目(No.20070246021) 复旦大学青年基金资助项目(No.EYF157003)
关键词 蛋白质14-3-3tau 滋养细胞 BEWO细胞 侵袭 ERK1/2信号通路 Protein 14-3-3 tau Trophocytes BeWo cells Invasiveness ERK1/2 signal pathway
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参考文献16

  • 1Goldman - Wohl D, Yagel S. Regulation of trophoblast invasion : from normal implantation to pre - eclampsia [ J ]. Mol Cell Endocrinol, 2002, 187(1 -2) :233 -238.
  • 2Hermeking H, Benzinger A. 14 - 3 - 3 proteins in cell cycle regulation[ J]. Semin Cancer Biol, 2006, 16(3):183 - 192.
  • 3Aitken A. 14 - 3 - 3 proteins: a historic overview[J].Semin Cancer Biol, 2006, 16(3) : 162 - 172.
  • 4Hoang VM, Foulk R, Clauser K, et al. Functional proteomics : examining the effects of hypoxia on the cytotrophoblast protein repertoire [ J ]. Biochemistry, 2001, 40 ( 13 ) :4077 -4086.
  • 5Park JK, Kang MY, Kim YH, et al. PKC delta in pre-eclamptic placentas promotes Bax dissociation from 14 -3 - 3 zeta through 14 - 3 - 3 zeta phosphorylation[ J ]. Placenta, 2008, 29(7) :584 -592.
  • 6Hu R, Jin H, Zhou S, et al. Proteomic analysis of hypoxia - induced responses in the syncytialization of human placental cell line BeWo [ J ]. Placenta, 2007, 28 ( 5 - 6) : 399 - 407.
  • 7Clokie SJ, Cheung KY, Mackie S, et al. BCR kinase phosphorylates 14 - 3 - 3 Tau on residue 233 [ J ]. Febs J, 2005, 272( 15 ) :3767 - 3776.
  • 8Jin H, Ma KD, Hu R, et al. Analysis of expression and comparative profile of normal placental tissue proteins and those in preeclampsia patients using proteomic approaches [J]. Anal Chim Acta, 2008, 629( 1 -2):158 -164.
  • 9Sullivan MH. Endocrine cell lines from the placenta[ J]. Mol Cell Endocrinol, 2004, 228 ( 1 - 2) : 103 - 119.
  • 10隋立荣,吕英志,孙立斌,王谦,杨凤英,李沈一.应用组织芯片研究E-cadherin在大肠癌的表达[J].中国病理生理杂志,2007,23(7):1439-1441. 被引量:6

二级参考文献15

  • 1车向明,王曙逢,王康,盛薇,刘晓毅.肿瘤细胞E-cadherin的表达与对基底膜浸润能力的关系[J].西安交通大学学报(医学版),2004,25(3):271-273. 被引量:3
  • 2李刚,王万里,魏莉.E-cadherin、β-catenin、FAK在大肠癌的表达及其意义[J].肿瘤防治研究,2005,32(9):542-544. 被引量:11
  • 3Nives PS. Tumor suppressor gene E - cadherin and its role in normal and malignant ceils [ J ]. Cancer Cell Int, 2003, 3(1): 17.
  • 4Takeichi M. Cadherins in cancer: implications for invasion and metastasis[J]. Curt Opin Cell Biol, 1993, 5 (5) : 806 -811.
  • 5Turel KR, Rao SGA. Expression of the cell adhesion molecule E - cadherin by the human bone marrow stromal cell and its probable role in CD34 ^+ stem cell adhesion [ J ]. Cell Biol Int, 1998, 22(9 - 10) : 641 -648.
  • 6Corn PG, Smith BD, Ruckdeschel ES, et al. E - cadherin expression is silenced by 5 ' CpG island methylation in acute leukemial [ J ]. Clin Cancer Res, 2000, 6 ( 11 ) : 4243 - 4248.
  • 7Miki I, Ishihara N, Otoshi M, et al. Simple colorimetric cell - cell adhesion assay using MTT - stained leukemia cells[J]. J Immunol Methods, 1993, 164 (2) : 255 - 261.
  • 8Cavallaro U, Schaffhauster B, Christofori G. Cadherins and the tumour progression : is it all in a switch? [ J ]. Cancer Lett, 2002, 176(2) : 123 - 128.
  • 9Taichman RS. Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem - cell niche [J].Blood, 2005, 105(7) : 2631 -2639.
  • 10Zhou J, Mauerer K, Farina L, et al. The role of the tumor microenvironment in hematological malignancies and implication for therapy[J]. Front Biosci, 2005, 10(5) : 1581 - 1596.

共引文献12

同被引文献15

  • 1金英,范莹,闫恩志,宗志红,包翠芬,李智.MAP激酶家族在淀粉样β蛋白片段25~35引起的大鼠海马炎症反应及细胞凋亡中的作用(英文)[J].中国药理学与毒理学杂志,2005,19(3):161-168. 被引量:11
  • 2Abe J, Kusuhara M, Ulevitch R J, et al. Bigmitogen-activated protein ki-nase 1 (BMK1) is a redox-sensitive kinase [J]. J Biol Chem, 1996 ; 271 (28) : 16586-90.
  • 3Toumier C, Whitmarsh A J, Cavanagh J, et al. Mitogen-activated proteinkinase 7 is an activator of the c-Jun NH2-terminal kinase[J].Proc NatlAead Sei USA,1997 ;94(14) :7337-42.
  • 4Raingeaud J, Whitmarsh AJ, Barrett T,et al. MKK3-and MKK6-regulatedgene expression is mediated by the p38 mitogen-aetivated protein kinasesignal transducion pathway [J].Mol Cell Biol, 1996 ; 16 (3) : 1247-55.
  • 5Jiang Y, Chen C, Li Z,et al. Characterization of the structure and functionof a new mitogen-activated protein kinase (p38β)[J]. J Biol Chem,1996 ;271 (30) : 17920-6.
  • 6Mennin T, De Paola M, Bigini P,et al. Nonhematopoietic erythropoietinderivatives prevent motoneuron degeneration in vitro and in vivo[J].Mol Med,2006;12(7-8) :153-60.
  • 7Avrarnovich Y, Amit T, Youdim MB. Non-steroidal anti-inflammatorydrugs stimulate secretion of non-amyloidogenic precursor protein [J].JBiol Chem ,2002 ;277 (35) :31466-73.
  • 8Morishima Y, Gotoh Y, Zieg J, et al. Beta-amyloid induces neuronal ap-optosis via a mechanism that involves the c-Jun N-terminal kinase path-way and the induction of Fas ligand[J].J Neurosci, 2001 ; 21 (19) :7551-60.
  • 9Shoji M ,Iwakami N, Takeuchi S, et al. JNK activation is associate withintracellular beta-amyloid accumulation [J]. Brain Res Mol Brain Res,2000;85 (1-2) :221-33.
  • 10Savage MJ, Lin YG, Ciallella JR, et al. Activation of c-Jun N-terminalkinase and p38 in an Alzheimer's disease model is associated with amy-loid deposition[J].J Neurosci,2002;22(9) :3376-85.

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