摘要
应用紫外分光光度法和荧光光度法研究了3,5-二溴水杨醛缩牛磺酸席夫碱(DSTSB)在pH3.2的介质中,与牛血清白蛋白(BSA)的相互作用(结合作用),考察了DSTSB对BSA构象的影响。讨论了DSTSB对BSA内源荧光的淬灭机理,测得猝灭常数Kq=2.1×1014L.mol-1.s-1,结合常数(KA=6.31×108L.mol-1)和结合点位数(n=1.1725)。依据Frester辐射能量转移理论确定了荧光给体-受体间距离(r=3.67nm)。
The interaction beween 3, 5-dibromo-salicylaldehyde-taurine Schiff base （DSTSB） and bovine serum albumin （BSA） in aqueous solution of pH 3. 2 was studied by UV spectrophotometry and fluorospectrophotometry. Effect of DSTSB on the configuration of BSA was also studied. Mechanism of quenching of fluorescence of BSA by DSTSB was discussed. Quenching constant （Kq）, equilibrium constant of combination （KA） and number of combination site （n） were determined and values obtained were 2. 1 ×1014L·mol-1·s-1, 6. 31×108L·mol-1 and 1. 172 5 respectively. By the F6rester radiating energy transfer theory, the value of combination distance between the donor and acceptor was found to be 3. 67 nm.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2010年第3期270-272,275,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
荧光光度法
荧光猝灭
席夫碱
牛血清白蛋白
Fluorospectrophotometry
Fluorescence quenching
Schiff base
Bovine serum albumin
