摘要
目的了解浙江省伤寒副伤寒沙门菌耐药及分子分型特点,为伤寒副伤寒防制工作提供依据。方法采用WHO推荐的改良K-B纸片法,对209株甲型副伤寒沙门菌和5株伤寒沙门菌进行14种抗生素敏感性试验;运用脉冲场凝胶电泳分型(PFGE)方法对119株甲型副伤寒沙门菌进行分子分型及流行病学特征分析。结果209株甲型副伤寒沙门菌对红霉素、萘啶酸和利福平100%耐药,对阿米卡星100%敏感,对环丙沙星的敏感率仅为58%;5株伤寒沙门菌对红霉素和利福平100%耐药,对头孢噻吩、头孢噻肟、庆大霉素、环丙沙星、氨苄西林、阿米卡星和阿莫西林/克拉维酸100%敏感;不同地区间的甲型副伤寒沙门菌对大部分药物敏感率一致,只有多西环素存在较大差异;119株甲型副伤寒沙门菌共产生20种PFGE带型,并主要集中在2种同源性较高的型别。结论2008年浙江省伤寒副伤寒以甲型副伤寒菌株为优势株,不同地区甲型副伤寒沙门菌对大部分抗生素敏感率一致;高发地区(台州)的菌株PFGE带型比较分散,可能与菌株变异有关。
Objective To know antibiotic resistance and molecular types of Salmonella isolated in Zhejiang province and to provide evidence for typhoid fever and paratyphoid fever prevention.Methods K-B method recommended by WHO was used to detect 209 strains of paratyphi A and 5 strains of typhi and 119 isolates were classified by pulsed-field gel electrophorese（PFGE）.Results Overall 209 strains of paratyphi A were sensitive to amikacin and all strains were resistant to erythromycin,nalidixic acid and rifampicin.The sensitive rate to ciprofloxacin of 209 strains of paratyphi A was only 58%.Five strains of typhi were sensitive to cephalothin,cefotaxime,gentamycin,ciprofloxacin,ampicillin,amikacin,and amoxicillin clavulanate and all strains were resistant to erythromycin and rifampicin.The sensitive rates of the strains isolated in different cities were in concordance with the most of antibiotics,except doxycycline.A total of 119 isolates were classified to 20 types by PFGE.Conclusion Most of paratyphi A isolated in Zhejiang province are classified to two types.There is no difference in antibiotic resistance rate of paratyphi A among strains isolated in different areas.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第3期328-330,共3页
Chinese Journal of Public Health
基金
浙江省"钱江人才"科技计划(2007R10018)
关键词
伤寒
副伤寒
耐药率
脉冲场凝胶电泳
typhoid fever
paratyphoid fever
resistant rate
pulsed-field gel electrophorese（PFGE）
