摘要
目的:研究急性低氧及力竭运动对大鼠骨骼肌成肌调节因子MyoD和myogenin基因表达的影响。方法:取健康SD雌性大鼠80只,随机分成四大组:1)常氧对照组(C);2)常氧力竭组(NE);3)低氧对照组(HC);4)低氧力竭组(HE)。采用半定量反转录聚合酶链式反应(RT-PCR)法,测定各组骨骼肌成肌调节因子MyoD和myogenin的mRNA基因表达。结果:力竭运动(NE组)引起的MyoD及myogenin基因表达最为明显(P<0.01),在力竭后12 h达峰值。单纯低氧条件下(HC组)3 d后,可引起MyoD基因表达明显升高(P<0.05);而低氧暴露加力竭运动后(HE组),则myogenin基因表达明显升高。结论:提示成肌调节因子,特别是MyoD及myogenin在运动及低氧导致的骨骼肌损伤及修复中起着重要作用。
The paper is to investigate the influences of acute hypoxia and exhaustive exercise on the gene expression of myogenic regulatory factors（MRFs） of skeletal muscle in rats.80 female SD rats are randomly divided into four groups： 1） Control at normoxic condition（C）,2） Exhausting at normoxic condition NE）,3） Control at hypoxic condition（HC）,4） Exhausting after hypoxic condition（HE）. The gene expression of skeletal muscle Myo-D and myogenin is determined by using the semi-quantitative reverse transcription PCR technology.Results：The mRNA of Myo-D and myogenin are found to be significantly greater for NE compared with control.The peaks are at 12h post exhaustion.When the gene expressions of skeletal muscle Myo-D under hypoxic condition（HC） increased significantly,the ones of myogenin in rats exhausted after hypoxic condition（HE） also upregulated.In conclusion,MRFs,especially Myo-D and myogenin,play an important role in muscle regeneration after injury induced by exercise and hypoxia.
出处
《北京体育大学学报》
CSSCI
北大核心
2010年第1期45-47,共3页
Journal of Beijing Sport University
基金
江苏省教育厅自然科学基金课题(07KJD180242)
扬州大学自然科学基金课题(批准号:2006XJJ10)
