摘要
黑龙江省完达山东部林区是东北马鹿(Cervus elaphus xanthopygus)种群密度较高的分布区之一,本文对2年冬季采集的167份马鹿粪便进行了7个微卫星座位的个体识别,评价了马鹿种群遗传多样性,并分析近期马鹿数量的急剧下降对种群遗传结构的影响。结果表明:167份粪便DNA分属66只个体;种群平均等位基因数9.00±2.77;平均有效等位基因数3.97±0.99;平均多态信息含量0.69±0.09;平均期望杂合度0.74±0.08;平均观察杂合度0.69±0.08。整个种群显著偏离Hardy-Weinberg平衡,但种群固定系数Fis为0.060,没有显著偏离零。可见,完达山东部林区马鹿种群遗传多样性较高,数量的急剧下降近期还没有表现在种群遗传结构上。
The eastern Wandashan Mountains of Heilongjiang Province is one of the areas with high population density of wapiti（Cervus elaphus xanthopygus）.In this paper,167 wapiti fecal samples were collected there in two winters to identify their DNA based on 7 microsatellite loci,aimed to study the genetic diversity of the population,and the effects of the population＇s dramatic decline in recent years on the population genetic structure.The DNA of the samples was assigned to 66 individuals.The average numbers of the alleles and effective alleles were 9.00±2.77 and 3.97±0.99,respectively,the average polymorphism information content was 0.69±0.09,and the average observed and expected heterozygosity values were 0.69±0.08 and 0.74±0.08,respectively.The population was significantly deviated from Hardy-Weinberg Equilibrium,but the inbreeding coefficient was 0.060,not significantly deviated from zero.It was suggested that the genetic diversity of the population was still higher,and the dramatic decline of the population didn＇t yet affect the population genetic structure due to time lag.
出处
《生态学杂志》
CAS
CSCD
北大核心
2010年第3期543-548,共6页
Chinese Journal of Ecology
基金
国家自然科学基金项目(30870309)
黑龙江省自然科学基金重点项目(ZJN-0501)
美国老虎和犀牛基金资助项目(98210-2-G191)
关键词
完达山东部
东北马鹿
遗传多样性
微卫星
Eastern Wandashan Mountains
wapiti（Cervus elephus xanthopygus）
genetic diversity
microsatellite
