摘要
目的:探讨人参属4种药材人参、西洋参、三七、竹节参的鉴别方法及其系统发育关系。方法:扩增线粒体nad1基因,测序比对,分析该基因序列的差异。结果:获得人参(移山参、高丽参)和竹节参该基因的1290 bp片段序列,西洋参和三七的该基因片段长度则分别为1269 bp和1522 bp,主要差异为该基因的b/c内含子。在NJ系统发育树上,人参和竹节参关系最近,其次是西洋参,而与三七的关系最远。结论:基于该片段的碱基差异可从这4种药材中将西洋参、三七鉴别出来。人参属植物线粒体nad1基因b/c内含子存在一定的进化信息,可以进一步用于人参属药材的鉴别及系统发育关系分析。
Objective：To study the identification method and phylogenetic relationships of four medicines of Panax L.genus：Panax ginseng C.A.Meyer,P.quinquefolicum L.,P.notoginseng（Burk.）F.H.Chen and P.japonicus C.A.Meyer.Methods：The mitochondrial nad 1 gene was amplified.Their sequence differences were analyzed after sequencing and alignment.Results：The sequence lengthes of P.ginseng（including Chinese transplanted ginseng and Korean ginseng）,P.japonicus were 1 290 bp,and those of P.quinquefolicum and P.notoginseng were 1 269 bp and 1 522 bp respectively.The main difference among these sequences was in nad 1 gene b/c intron.The NJ phylogenetic tree showed that P.ginseng was most closely related to P.japonicus,next was closer to P.quinquefolicum,and P.notoginseng was comparatively distantly related to P.ginseng.Conclusion： P.quinquefolicum and P.notoginseng can be identified from the 4 medicines of Panax L.based on the sequence difference in mitochondrial nad 1 gene.The mitochondrial nad 1 gene b/c intron can provide some evolutionary information,therefore,it is useful to identify and phylogeneticly analyse for the medicines of Panax L.genus.
出处
《中药材》
CAS
CSCD
北大核心
2010年第1期45-48,共4页
Journal of Chinese Medicinal Materials
基金
上海市科学技术委员会中药现代化专项(08DZ1972500)
