摘要
以猕猴桃的顶芽作外植体进行离体培养，芽分化率最高，为７９．１％，其次是侧芽和茎段，两者的芽分化率分别可达４５．８％和２４．４％，叶片的芽分化率为０。以顶芽为外植体，在添加１．５ｍｇ／ＬＢＡＰ和０．２ｍｇ／ＬＮＡＡ，Ｎ素减半的改良ＭＳ培养基上进行芽分化和继代培养，芽分化率可达到９７．７％，在继代培养中补充１０ｍｇ／Ｌ的谷氨酸盐，可大大提高继代增殖系数β１，到第三次继代时，β１可达到１２７．５，而此时对照的的β１仅为１２．７。以Ｎ素减半或Ｎ素及Ｆｅ盐减半的ＭＳ培养基作生根培养基，生根率均可达１００％，比对照提高３０％，平均根长分别比对照长４．２ｃｍ和７．１ｃｍ，开始生根时间分别比对照早３ｄ和６ｄ；在生根培养基上添加３％的活性炭可以进一步提高生根长度，缩短生根时间。研究认为繁殖系数ε是衡量一个外植体可繁殖成多少苗木的主要指标，本试验中ε＝１０１，即一个外植体可繁殖１０１棵苗。
On the test of tissue culture,using shoot tips of Actinidia chinensis as explant,the highest rate of shoot differentiation is 79.1%,and the shoot differentiation rate of lateral shoot and stem are respectively 45.8% and 24.4%,and the shoot differentiation rate of blade is 0%. On the test of shoot differentiation and subculture,using shoot tips as explant,and the improved MS culture medium by increasing 1.5 mg/L BAP and 0.2 mg/L NAA,and half decreasing N element,the rate of shoot differentiation can reach 97.7%. It can obviously improve the coefficient β 1 of subculture to add 10 mg/L glutamine on the test of subculture,when it comes to the third time of subculture, β 1 can get to 127.5,however the check β 1 is only 12.7. Taking MS culture medium on half decreasing N element or N element and Fe salt as taking root culture medium,the rooting rate can get to 100%,which increases 30% more than the comparison. The average root length are respectively 4.2 cm and 7.1 cm longer and the rooting time are respectively 3 days and 6 days earlier than those of the comparison. Adding 3% active carbon to rooting culture medium can further increase the root length and shorten the rooting time. The research holds that reproductive coefficient ε is the main index to measure how many planting stock one explant can reproduce. It is ε =101 on this test,which means that one explant can reproduce 101 planting stock.
出处
《林业科学研究》
CSCD
北大核心
1998年第6期635-639,共5页
Forest Research
基金
日本政府无偿援助中国的专项技术合作项目
关键词
猕猴桃
组织培养
培养基
繁殖系数
繁殖能力
Actinidia chinensis tissue culture culture medium reproductive coefficient
