摘要
近２０ａ来已对２０个属７０余种竹子进行了离体培养研究。以侧芽、顶芽、成熟胚作外植体诱导愈伤组织，由愈伤组织制备悬浮细胞进行细胞悬浮培养，由悬浮细胞制备原生质体进行原生质体培养。竹子愈伤组织经不定芽途径或体细胞胚发生途径再生完整植株。通过芽尖培养增殖新生芽进行竹微繁殖，并获得脱病毒种苗。以芽为外植体增殖的新芽或组培再生苗经继代培养诱导竹试管开花结实。
species of bamboo in 20 genera were cultured in vitro in recent 20 years.Plant regeneration via somatic embryogenesis and adventitious shoot was achieved in callus cultures derived from explants excised axially buds,shoot apices or mature zygotic embryos.Liquid suspension culture cells of bamboo served as protoplasts donors,and high yields of viable protoplasts were obtained,and callus from protoplasts of suspension cultured bamboo cells were recovered. About 70 species of bamboo tested were successfully propagated in vitro . For nearly each species,multiple shoots were produced from axially buds on stem node segments cultured on MS medium containing BA. A few callus plant could be regenerated adventitiously from callus. In vitro flowering was induced on shoot developed from nodal explant taken from regenerated plants. The flowering of bamboo shoots of some species occurred in unison,but not that of other species.
出处
《林业科学研究》
CSCD
北大核心
1998年第6期640-646,共7页
Forest Research
基金
浙江省自然科学基金
关键词
竹子
愈伤组织
悬浮细胞
原生质体
离体培养
bamboo callus cell suspension protoplast micropropagation in vitro flowering
