摘要
本试验旨在研究日粮中添加不同水平的三聚氰胺(melamine,MEL)在鸭蛋中的沉积和消除规律。选用体重为(1.66±0.16)kg和产蛋率为(75.15±3.29)%的19周龄健康金定产蛋鸭756只,随机分成7个处理,每个处理设6个重复,每个重复18只。7种试验日粮的MEL添加水平分别为:0、1、2、5、25、50和100mg/kg。各处理分别连续饲喂含不同水平MEL的日粮21d(沉积试验),随后改喂基础日粮21d(消除试验),试验期共42d。于沉积和消除试验的第1～11天、第13天、第15天、第17天和第21天分别测定鸭蛋可食部分中的MEL和三聚氰酸(cy-anuric acid,CYA)含量。结果表明,0～5mg/kg的4个处理之间鸭蛋中MEL含量差异不显著(P>0.05),25～100mg/kg的3个处理之间差异极显著(P<0.01),且均极显著高于0～5mg/kg的4个处理(P<0.01);鸭蛋MEL含量(Y,mg/kg)与日粮MEL添加水平(X,mg/kg)的关系方程为:Y=0.0121X+0.0165(R2=0.9994),MEL由日粮到鸭蛋中的生物富集系数为0.0121;通过鸭蛋排出的MEL(Y,mg/d)与蛋鸭摄入的MEL(X,mg/d)拟合的曲线为:Y=0.2618X+0.0947(R2=0.9999),MEL由蛋鸭到鸭蛋的母体转移系数为0.2618。日粮MEL添加水平越高,鸭蛋中的消除半衰期越短,完全消除需要的时间越长;5、25、50和100mg/kg处理鸭蛋中的MEL含量(Y,mg/kg)随时间(X,d)消除方程分别为:Y=0.0802X-0.7477(R2=0.7775)、Y=0.3268X-1.1608(R2=0.9871)、Y=0.5912X-1.3403(R2=0.9922)和Y=1.2004X-1.3956(R2=0.9883),根据上述方程计算各处理鸭蛋中MEL完全消除时间分别为3.48、7.81、9.31和14.61d;鸭蛋中未检测到CYA。结果显示,MEL在产蛋鸭日粮中浓度越高,其鸭蛋中沉积量越大,消除时间越长。
The objective of this study was to investigate the effects of dietary melamine levels on the deposition and depletion rules of melamine in eggs of laying ducks. Seven hundred and fifty-six 19-week-old Jinding laying ducks with average body weight of （1.66± 0.16） kg and laying rate of （75.15 ±3.29） % were randomly divided into 7 groups with 6 replicates in each group and 18 ducks in each replicate. Each group was fed a corn-soybean meal diet supplemented with 0, 1, 2, 5, 25, 50, 100 mg/kg melamine, respectively. The trial was divided into two sub trials, which included a deposition trial, from day 1 to 21, and a depletion trial from day 22 to 42. The trial lasted for 42 days. The melamine and cyanuric acid contents of the egg white and yolk were determined at day 1-11, 13, 15, 17, and 21 of the two sub trials. The results showed that there was no significant difference of melamine contents in the eggs among 4 groups from 0 to 5 mg/kg （P〉0.05） ; melamine contents in the eggs of 3 groups from 25 to 100 mg/kg were significantly higher than those of 4 groups from 0 to 5 mg/kg （P〈 0.01）, and the differences among 3 groups of 25-100 mg/kg were extremely significant （P〈0. 01）. The relationship between melamine contents （Y） in the eggs and dietary supplemental levels （X） could be described as： Y = 0. 012 1X ＋ 0. 016 5 （ R^ 2 = 0. 999 4）. The higher of melamine contents in the eggs, the longer of the depletion time of half-life, but complete depletion needed more time. The melamine depletion equations of 5, 25, 50 and 100 mg/kg groups were Y = 0.080 2X^-0.7477 （R^2=0.7775）, Y=0.326 8X^-1.1608（R^2 =0.987 1）, Y= 0.591 2X ^-1.3403 （R^2 =0.992 2）, Y= 1.200 4X ^-1.3956（R^2 = 0. 988 3）, and the complete depletion time of the 4 groups was more than 3.48, 7.80, 9.31 and 14.61 days, respectively. Cyanuric acid had not been detected in the eggs. The results indicated that melamine contents were increased with the increasing dietary supplemental levels, and the depletion time was also increased with the increasing melamine contents in the eggs.
出处
《动物营养学报》
CAS
CSCD
北大核心
2010年第2期416-423,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
农业部饲料中三聚氰胺限量制定专项
国家科技支撑计划(2006BAD12B06)
关键词
三聚氰胺
鸭蛋
沉积
消除
Melamine
Eggs of laying ducks
Deposition
Depletion
