摘要
目的了解支气管肺癌活组织黏膜组织蛋白酶D(Cath D)、肺表面活性物质相关蛋白A(SP-A)和组织葡萄糖转运蛋白1(GLUT-1)的表达。方法支气管镜下钳取异常组织黏膜41例,经光镜病理确诊为支气管肺癌38例(其中小细胞肺癌10例,鳞状细胞癌28例),炎症3例。用SP免疫组织化学方法检测Cath D、SP-A和GLUT-1的表达。结果①Cath D:小细胞癌-6例(60%),++4例(40%);鳞状细胞癌-4例(14%),+2例(7%),++8例(28%),+++14例(50%)。小细胞癌与鳞状细胞癌对Cath D的表达有明显差异(P<0.01),后者明显高于前者。鳞状细胞癌分化程度不同,Cath D的表达有明显差异(P<0.05),分化程度越低,表达越高;②SP-A:+++3例(炎症),++20例(高分化鳞癌15例,中分化鳞癌5例),+6例(中分化鳞癌),-12例(小细胞癌10例,低分化鳞癌2例);③GLUT-1:小细胞癌-6例,+2例,+++2例;鳞状细胞癌-5例,+7例,++11例,+++5例;炎症+++3例。小细胞癌与鳞状细胞癌对GLUT-1的表达有明显差异(P<0.05),后者明显高于前者;不同分化程度的鳞状细胞癌,GLUT-1的表达无差异(P(0.05);炎症与鳞癌GLUT-1表达有明显差异(P<0.05)与小细胞癌则无差异(P(0.05)。结论 Cath D有助于鳞状细胞癌的诊断和分化程度的评估,SP-A可能有助支气管黏膜炎症与高恶度肿瘤组织的鉴别,GLUT-1在鳞状细胞癌有高表达,有助于鳞状细胞癌的诊断和鉴别诊断。
Objective To examine the expression of Cath D, SP - A and GLUT - 1 in patients with bronchiogenic carcinoma. Methods A total of 41 patients were included in the study, 10 of whom received the histological diagnosis of small cell lung cancer （SCLC） . The other 28 were squamous cell carcinoma （SC） and 3 were inflammation. And all the samples were taken from the patients＇ tunica mucosa bronchiorum through bronchofibroscope, then we detected the cathepsin D, SP - A and GLUT - following SP immunohis- tochemistry. Results ①Cath D： 6 samples （60%） in group SCLC were negtive expression （-6） , 4 （40%）were moderately positive （＋＋4）, 4 （14%） were negative （-4）, 2 （7%） were positive （＋2）, 8 （28%） were moderately positive （＋＋8） and 14 （50%） were intensive positive （ ＋＋＋ 14） in the other group. SCLC was significant different from SC in expressing cathepsin D （P 〈0.01 ） , and the latter group was obviously exceeded with the former one. At the same time, the level of expression depended on the degree of the squamous cell differentiation （ P 〈 0.05 ） ; ②SP - A ： ＋ ＋ ＋ for 3 （inflammatory） , ＋ ＋ for 20 （ 15 were high differentiation squamous cell carcinoma; others were middle differentiation squamous cell carcinoma） , ＋ for 6 （middle differentiation squamous cell carcinoma） and - for 12（ 10 were small cell carcinoma and 2 were poorly differentiated squamous cell carcinoma） were found;③GLUT - 1 ：strong positive was 10 including 3 inflammatory and 7 well differentiated squamous cell carcinoma; positive was 11 （squamous cell carcinoma） ; weakly posi- tive was 9 （2 of them were small cell carcinoma, others were quamous cell carcinoma） ; negative was 6 （small cell carcinoma）. Conclu- sion The detection of cathepsin D is of great clinical significance in diagnosing and evaluating differentiation - degree in squamous cell carcinoma. The SP - A may contribute to identification of the tissue belong to bronchial mucosa. The high expression of GLUT - 1 in SC is of great clinical significance in tumor diagnosis.
出处
《医学研究杂志》
2010年第3期70-73,共4页
Journal of Medical Research
基金
吉林省教育厅"十一五"科学技术研究项目(吉教科字2007第244号)
