摘要
目的建立变应性鼻炎（allergic rhinitis，AR）小鼠模型，研究金黄色葡萄球菌肠毒素B（SEB）和卵清蛋白（OVA）在建立AR模型中的作用。方法将40只6～8周Balb／c小鼠随机分为4组：OVA组、SEB组、OVA＋SEB组、生理盐水组，并建立小鼠AR模型。采用析因设计分析各组小鼠临床症状评分、血清IgE和IL-4的水平，并对鼻黏膜行组织形态学观察。结果OVA组、SEB组、OVA＋SEB组及生理盐水组小鼠的症状评分分别为：0．90±0．99、0．70±0．82、6．80±1．03、0．60±0．70．OVA、SEB交互效应P〈0．01，有统计学意义，且OVA＋SEB组鼻黏膜出现明显的AR形态学改变。血清IgE、水平分别为25．41±3．87ng／ml、20．11±2．23ng／ml、122．72±5．68ng／ml、18．67±2．00ng／ml；IL-4的水平分别为27．37±1．52pg／ml、24．05±2．13pg／ml、63．73±3．81pg/ml、23．50±2．62pg/ml，二者OVA、SEB交互效应均有统计学意义。结论只有SEB和OVA协同作用后可致小鼠AR，即SEB可以提高机体对OVA的易感性，发挥免疫佐剂的作用。
Objective To establish mice modle of allergic rhinitis（AR） and to study the role of Staphylococcal enterotoxin B （SEB） and ovalbumin（OVA） in the modle. Methods Forty Balb/c mice were evenly randomized into OVA group , SEB group, OVA ＋ SEB group and normal sodium group and AR modle was established. The symptom scores,total serum IgE concentration, IL- 4 concentration were analyzed by factorial design. Meanwhile, the morphology change of nasal mucosa was observed. Results The symptom scores in OVA group, SEB group, OVA ＋ SEB group and normal sodium group were 6.80 ± 1.03,0.90 ±0.99,0.70 ± 0.82,0.60 ± 0.70 respec-tirely. The interaction of OVA and SEB had statistical significance（P 〈0. 01 ）. Nasal mucosa of OVA ＋ SEB group displayed an AR morphology change . Meanwhile, Total serum IgE concentrations were 25.41 ± 3. 87μg/ml, 20.11 ± 2.23 μg/ml, 122.72 ± 5.68 μg/ml, 18.67 ± 2. 00μg/ml respectirely. IL- 4 concentration were 27.37 ±1.52pg/m1,24.05 ±2. 13pg/ml, 63.73 ± 3.81pg/ml,23.50 ± 2.62pg/ml respectirely, the interaction of OVA and SEB in IgE and IL - 4 concentration had statistical significance too. Conclusion Only OVA and SEB cooperation could induce AR development. SEB can elevate the affectivity of OVA to the organism and play a role of immunity adjuvant.
出处
《医学研究杂志》
2010年第3期101-104,共4页
Journal of Medical Research
