摘要
使用超高效液相色谱-电喷雾串联四极杆质谱(UPLC-ESI-MS/MS)在多反应离子监测(MRM)模式下分离鉴定了烟草中7种潜香物质。烟样经甲醇提取,XAD-2柱分离纯化,选择中性丢失162u扫描确定母离子。通过对母离子二级质谱扫描(MS2),并根据m/z192.7、179.2、163.1、134.7、133.2等特征碎片离子峰分析结果确定了7种潜香型物质,分别为熊果苷、香豆酸-葡萄糖苷、香豆素奎尼酸、新绿原酸、绿原酸、葡萄糖基阿魏酸、3-氧化紫罗兰醇葡萄糖苷。
A method for identification of 7 aroma precursors in tobacco was established by using ultra performance liquid chromatography-tandem mass spectrometry interfaced with electro-spray ionization （UPLC-ESI-MS/MS） in the multiple reaction monitoring （MRM） mode. Aroma precursors in tobacco were extracted with methanol, cleaned up with XAD-2 column, then detected and identified by ESI-MS/MS with the neutral loss method. According to the characteristic product ions of m/z 192.7, 179.2, 163.1, 134.7, 133.2 acquired by product ion scan（MSZ）, 7 potential flavor compounds are identified, they are arbutin, trans-p-coumaric acid-4-o-β-D-glucopyrannoside, coumaroylquinic acid, neochlorogenic acid,chlorogenic acid, feruloylglycoside, 3-oxo-α-ionol-β-D-glycoside. The method is rapid, convenient and suitable for the determination of aroma precursors in tobacco leaves.
出处
《质谱学报》
EI
CAS
CSCD
2010年第2期88-93,共6页
Journal of Chinese Mass Spectrometry Society
关键词
烟草
潜香物质
超高效液相色谱-质谱联用
tobacco
aroma precursors
ultra performance liquid chromatography-tandem mass spectrometry interfaced with electro-spray ionization（UPLC-MS/MS）
