摘要
[Objective]The aim was to study cold-resistant indexes and physiological characters of three different cold-resisitant tomato strains selected under natural conditions. [Method]With 3-4 weeks tomato seedlings as tested materials,through simulating external environment conditions by the cold treatment in lab,the survival ratio and growth status of seedlings were identified; by studying the morphological change of in vitro leaves after cold treatment and determining the relative conductivity,the cold-resistant degree of different tomato strains was identified and the correlation between conductivity and survival rate was analyzed; the variation of soluble sugar and praline content in plant of tomato was determined under the condition of cold stress. [Result]The results showed that leaf morphological changes,size of conductivity and growth status of different tomato strains after cold treatment have significant differences; the variation of soluble sugar contents in plant has significant differences,while the variation of proline content has no significant differences. [Conclusion]There is a certain correlation between conductivity and survival rate under cold treatment conditions,and plant cold tolerance is related to accumulation of soluble sugar content.
[目的]研究自然条件下选育的3种不同耐寒番茄品系的耐寒指标及其生理特性。[方法]以3～4周的番茄幼苗为材料,通过实验室冷处理模拟外界环境条件,鉴定植株的存活及生长状况;研究植株离体叶片冷处理恢复后的形态变化并测定相对电导率,鉴定不同品系番茄植株的耐寒程度,分析电导率与存活率的相关性;测定冷胁迫条件下番茄植株体内可溶性糖和脯氨酸含量变化。[结果]正常培养条件下,耐寒品系番茄生长4周的植株形态矮小,中等耐寒品系番茄形态略高,不耐寒品系最为高壮。-4℃条件下处理6h后,耐寒品系、中等耐寒品系、不耐寒品系番茄,平均存活率分别达63.33%、44.57%、1.23%。番茄离体叶片-4℃冷处理后,耐寒品系叶片卷缩程度较小,叶片颜色呈碧绿色;中等耐寒品系叶片颜色呈深绿色且卷缩程度较大;不耐寒品系叶片颜色呈深黑色,卷缩程度最大。耐寒品系90%以上的相对电导率在42.62%以下,不耐寒品系90%以上的电导率在62.67%以上,中等耐寒品系90%以上的电导率约为52.05%。不同品系番茄植株体内可溶性糖含量变化差异显著,脯氨酸含量变化差异不显著。[结论]冷处理条件下电导率与存活率存在一定相关性,植株耐寒性与可溶性糖含量的积累有关。
基金
Supported by National Key Technology R&D Program of China(2008BADC0B00)
Natural Innovation Experiment Program for University students (081035917)
International Cooperation Program of Anhui (08080703002)~~
