Micropropagation of Three Bamboo Species from Nodal Explants of Mature Culms
Micropropagation of Three Bamboo Species from Nodal Explants of Mature Culms
摘要
A micropropagation technique is developed for the multiplication of Dendrocalamus strictus （D. strictus）, Dendrocalamus asper （19. asper） and Bambusa bambos （B. bambos） through shoot proliferation. Nodal explants obtained from field gown clumps were used to initiate cultures. Shoots were induced on Murashige and Skoog＇s （MS） medium supplemented with 5 mg L^-1 6-benzylamino purine （BAP）. Rapid shoot multiplication was obtained on MS medium containing 3 mg Lt BAP in D. asper, B. bambos and 2 mg L^-1 BAP in D. strictus. In vitro multiplied shoots showed best root induction on half strength MS supplemented with 1 mg L^-1 indole-3 butyric acid （IBA） and 0.5 mg L^-1 1-naphthalene acetic acid （NAA） in D. asper. Pre-rooting conditioning followed by culturing on half strength MS supplemented with 1 mg L^-1 IBA and 2 mg L^-1 IBA showed maximum root induction in D. strictus and B. bambos, respectively. Further root proliferation was obtained on hormone free medium. The micropropagated plantlets were acclimatized and successfully transferred on soil in green house.
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