摘要
【目的】采用双波长法同时测定谷类和豆类作物籽粒中直链和支链淀粉的含量。【方法】由直链淀粉一碘、支链淀粉一碘复合物的吸收光谱，确定直链和支链淀粉的波长对分别为495、565、530和654nm，将其代入回归方程，可求出样品中直链和支链淀粉的含量。【结果】直链淀粉的浓度在0～80mg／g、支链淀粉的浓度在0～400mg／L有很好的线性关系，即符合比耳定律。【结论】双波长法可以有效地消除吸收池和试剂等因素引起的误差，具有准确度高等特点，用该方法测定直链和支链淀粉的含量得到了很好的结果。
[ Objective ] The paper mainly uses dual - wave length spectrophotometry method to study the determinnation of amylose and amylopectin in grain and bean. [Method]Based on the absorption spectra of the two complexes,the amylose- wave length of 495/565 nm,and amylopectin wave length of 530/654 nm were obtained as the measuring wave length. According to the linear regression equationes, the paper can determine the content of amylose and amylopectin in sample solution. [ Result] The result shows that amylose concentration is between 0 - 80 mg/L and amylopectin concentration is between 0 - 400 mg/L. [ Conclusion ] The content of amylose and amylopeetin determined by the method is accurate, because it is not influenced by the reagent and the error which is caused by the absorption cell . So that it is most suitable to determine amyloy and amylopeetin and the results of the experiments is most satisfactory.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2010年第3期564-568,共5页
Xinjiang Agricultural Sciences
基金
霍英东教育基金资助项目(111019)
关键词
双波长法
含量测定
直链淀粉
支链淀粉
dual - wavelength spectrophotometry
content determination
amylose
amylopectin
