摘要
从市售的木聚糖酶、淀粉酶、纤维素酶、中性蛋白酶、复合植物水解酶、果胶酶中筛选提取光皮树油的水解酶,实验结果得到纤维素酶对光皮树果实的提油作用最强,其提油率达65.29%,复合植物水解酶次之,为64.05%,木聚糖酶效果最差,仅为54.47%。通过单因素实验得到纤维素酶提取光皮树油的最适工艺条件为:酶解pH 5.8,料液比1∶3,酶加量2.5%,酶解温度40℃,酶解时间4h。该条件下,油的乳化率低,提出来的基本上是清油,且提油率达76.64%。
The optimum enzyme of extracting oil from Cornus wilsoniana fruit was selected from xylanase,amylase,cellulase,neutral protease,composite plant hydrolytic enzymes and pectinase.The results showed that the oil extraction rate（65.29%） with cellulase was the highest among the six kinds of enzyme,and the oil exraction rate（64.05%） with composite plant hydrolytic enzymes was the second,the lowest oil extraction rate was 54.47% only by xylanase.By single factor test,the optimum conditions with cellulase was obtained as follows： pH 5.8,solid-liquid ratio 1∶3,enzyme dosage 2.5%,reaction temperature 40 ℃,reaction time 4 h.Under these conditions,the oil extraction rate reached 76.64%.
出处
《中国油脂》
CAS
CSCD
北大核心
2010年第3期6-9,共4页
China Oils and Fats
基金
国家科技部科技支撑项目<生物柴油绿色高效催化剂的研究>(2007BAD41B04)
生物柴油清洁生产工艺研究与示范(2007BAD41B05)
关键词
光皮树油
水酶法
纤维素酶
筛选
Cornus wilsoniana fruit oil
aqueous enzymatic extraction
cellulase
selection
