摘要
目的:建立高效液相法同时测定淫羊藿饮片中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷和宝藿苷I的含量,比较37批次淫羊藿饮片中5种黄酮类成分含量差异。方法:应用高效液相法进行测定:色谱柱为Luna-C18(4.6mm×250mm,5μm);乙腈-水梯度洗脱,0～29min,乙腈25%;29～30min,乙腈25%～41%;30～55min,乙腈41%;55～56min:乙腈41%～25%;56～64min,乙腈25%;流速为1mL·min-1;检测波长为270nm;进样量为10μL;柱温为30℃。结果:37批次淫羊藿饮片中朝藿定A含量在1.80～3.88mg·g-1,朝藿定B含量在3.48～20.25mg·g-1,朝藿定C含量在1.37～29.62mg·g-1,淫羊藿苷含量在3.45～8.12mg·g-1,宝藿苷I含量在1.52～5.23mg·g-1。其中以柔毛淫羊藿中朝藿定C含量最高,淫羊藿中朝藿定C、朝藿定B含量最高。结论:本实验中所建立的色谱方法可以简便、准确测定淫羊藿饮片中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷和宝藿苷I的含量,重复性好;建议在建立淫羊藿饮片的质量标准时,应建立多种黄酮类成分指标评价体系,以全面控制淫羊藿饮片的质量。
OKIECTIVE An HPLC method was developed to determine the content of epimedin A, epimedin B, epimedin C, icariin and Baohuoside 1, To compare the content of the main flavonoids in 37 batches Epimedium decoction pieces. METHODSAn HPLC method was used. The samples were separated on an LunmC18（4. 6 mm× 251） mm, 5 pro）column and were eluted with acetonitrile-water （0-29 min, acetonitrile 25%; 29～30 min, acetonitrile 25～41%;30-55 min, acetonitrile 41 %;55-- 56 min, acetonitrile 41-25% ; 56-64 min, acetonitrile 25%） ; Flow rate was 1.0 mL/min. The detection wavelength was set at 270 nm. Column temperature was 30℃. RESULTS The content of epimedin A, epimedin B, epimedin C, icariin and Baohuo- side I was 1.88 - 3. 88 mg/g, 3. 48 - 20. 25 mg/g, 1.37 - 29. 62 mg/g, 3. 45 8. 12 mg/g and 1.52 - 5.23 mg/g respectivdly. The content of epimedin B in Epimedium brevicomu Maxim. is more than the others, the content of epimedin C in Epimedium pubescens Maxim. is more than the others. CONCLUSION The method for quantization of epimedin A, epimedin B, epimedin C, icariin and Baohuoside I in Epimedium decoction pieces is accurate and reliable, which can be used to evaluate the quality of Epimedium decoction pieces. The results suggest that we should establish multiple indicators of flavonoids to achieve a full control of the quality of Epimedium decoction pieces.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2010年第6期449-452,共4页
Chinese Journal of Hospital Pharmacy
基金
中国药典2010年版一部品种质量标准修订项目(编号:YS-240)
国家自然基金资助项目(编号:30572372
30973944)
